冠通方对大鼠心梗后心功能不全心肌细胞凋亡及病理形态的影响  

Effects of Guantong Formula on Apoptosis and Pathological Morphology of Cardiac Insufficiency Myocardial Cells in Rats after Myocardial Infarction

作　　者：韦斌[1] 莫雪梅 王强 叶星江[4] 林洪升[4] 卢健棋[4] 姜浩[4] WEI Bin;MO Xue-mei;WANG Qiang;YE Xing-jiang;LIN Hong-sheng;LU Jian-qi;JIANG Hao(Ruikang Hospital affiliated to Guangxi University of Chinese Medicine,Nanning Guangxi 530011;Guangxi ArmedPoliceForceHospital,Nanning Guangxi 530003;Guangxi InternationalZhuang MedicalHospital,Nanning Guangxi 530201;First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning Guangxi 530023)

机构地区：[1]广西中医药大学附属瑞康医院,广西南宁530011 [2]武警广西总队医院,广西南宁530003 [3]广西国际壮医医院,广西南宁530201 [4]广西中医药大学第一附属医院,广西南宁530023

出　　处：《广西中医药大学学报》2019年第4期5-9,共5页Journal of Guangxi University Of Chinese Medicine

基　　金：广西高校中青年教师科研基础能力提升项目(编号:KY2015LX163)

摘　　要：[目的]研究观察冠通方对大鼠心梗后心功能不全心肌细胞的凋亡及病理形态的影响,探讨冠通方对心肌细胞的保护作用。[方法]24只SD雌性大鼠随机分为假手术组、模型组、冠通方组、西药组,每组6只。各组手术造模后,假手术组、模型组予生理盐水灌服,冠通方组予中药冠通方灌服,西药组予卡托普利灌服,各组均连续灌胃4周后,取出心脏制备左室心肌病理切片。观察各组病理切片在光镜下心肌细胞病理形态学结构变化,应用原位末端标记法(TUNEL)检测判定细胞凋亡,免疫组化法检测大鼠心肌Bcl-2,Fas等蛋白的表达。[结果]冠通方组心肌细胞在光镜下的结构形态得到较好的保护,冠通方组的细胞凋亡指数较模型组显著下降(P<0.05),与模型组比较,Bcl-2蛋白表达明显升高(P<0.05),Fas蛋白表达显著下降(P<0.05)。[结论]冠通方可以减少心梗后大鼠心衰心肌细胞的凋亡,从而达到保护心肌细胞的作用。[Objective]To observe the effects of Guantong Formula on the apoptosis and pathological morphology of cardiac insufficiency myocardial cells after myocardial infarction in rats, and to discuss the protective effect of Guantong Formula on myocardial cells.[Methods]24 female SD rats were randomly divided into a sham operation group, a model group, a Guantong formula group, and a western medicine group. There were 6 rats in each group. After surgical modeling in each group the sham operation group and the model group were gavaged with saline, the Guantong formula group was gavaged with the Guantong formula, and the western medicine group was gavaged with captopril. After 4 weeks of gastric perfusion the heart was removed to prepare a left ventricular myocardial pathological section. The pathological and morphological changes of myocardial cells of each group were observed under light microscope. TdT-mediated dUTP nick end labeling(TUNEL)method was used to determine the apoptosis. The immunohistochemical method was used to detect the expression of Bcl-2, Fas and other proteins in rat cardiac muscle.[Results]Under light microscope the structure and morphology of myocardial cells in the Guantong formula group were better protected. The apoptosis index of the Guantong formula group was significantly lower than that in the model group(P<0.05). Compared with the model group the Bcl-2 protein significantly increased(P<0.05),and Fas protein expression decreased significantly(P<0.05).[Conclusion]Guantong Formula can be applied to reduce the myocardial cell apoptosis after myocardial infarction and protect the myocardial cells.

关 键 词：心肌梗死 心力衰竭 细胞凋亡 冠通方 心肌细胞 实验研究 

分 类 号：R542.22[医药卫生—心血管疾病]