饲料碳水化合物水平对斜带石斑鱼生长性能、体成分、血浆生化指标及肠道和肝脏酶活性的影响  

Effects of Dietary Carbohydrate Level on Growth Performance,Body Composition,Plasma Biochemical Parameters and Intestinal and Liver Enzyme Activities of Orange-Spotted Grouper(Epinephelus coioides)

作　　者：刘浩 杨俊江 董晓慧 谭北平 杨奇慧 迟淑艳 刘泓宇 章双 杨原志 LIU Hao;YANG Junjiang;DONG Xiaohui;TAN Beiping;YANG Qihui;CHI Shuyan;LIU Hongyu;ZHANG Shuang;YANG Yuanzhi(Laboratory of Aquatic Animal Nutrition and Feed,College of Fisheries,Guangdong Ocean University,Zhanjiang 524088,China;Aquatic Animals Precision Nutrition and High-Efficiency Feed Engineering Research Center of Guangdong Province,Zhanjiang 524088,China)

机构地区：[1]广东海洋大学水产学院,水产动物营养与饲料实验室,湛江524088 [2]广东省水产动物精准营养与高效饲料工程技术研究中心,湛江524088

出　　处：《动物营养学报》2020年第1期357-371,共15页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：现代农业产业技术体系专项(CARS-47);广东海洋大学“冲一流(231419011)”省财政专项资金建设项目

摘　　要：本试验旨在研究饲料碳水化合物水平对斜带石斑鱼生长性能、体成分、血浆生化指标及肠道和肝脏酶活性的影响。选择健康、初重(102.80±1.02)g的斜带石斑鱼300尾,随机分为5个组,每组3个重复,每个重复20尾。5组分别投喂碳水化合物水平为7%、14%、21%、28%和35%的等氮等能的试验饲料。试验期8周。结果表明:1)28%组的特定生长率(SGR)、增重率(WGR)显著高于其他各组(P<0.05),28%组的饲料系数(FCR)显著低于7%和14%组(P<0.05),28%和35%组的蛋白质效率(PER)、肥满度(CF)和肝体比(HSI)显著高于7%组(P<0.05),35%组的脏体比(VSI)显著高于7%组(P<0.05)。2)7%组的干物质表观消化率显著低于21%、28%和35%组(P<0.05)。35%组的粗脂肪和能量表观消化率显著低于7%、14%和21%组(P<0.05)。3)28%和35%组的全鱼粗蛋白质含量显著低于7%组(P<0.05),35%组的全鱼粗脂肪含量显著高于其他各组(P<0.05),35%组的全鱼粗灰分含量显著小于7%、14%和28%组(P<0.05)。28%和35%组的肌肉粗脂肪含量显著高于7%组(P<0.05),7%组的肌肉粗灰分含量显著高于其他各组(P<0.05)。35%组的肝糖原含量显著高于7%、14%和21%组(P<0.05),35%组的肌糖原含量显著高于其他各组(P<0.05)。4)7%组的血浆总蛋白(TP)含量显著低于其他各组(P<0.05),21%、28%和35%组的血浆葡萄糖(GLU)含量显著高于7%和14%组(P<0.05),28%和35%组的血浆甘油三酯(TG)含量显著高于7%和14%组(P<0.05),28%和35%组的血浆总胆固醇(TC)含量显著高于7%、14%和21%组(P<0.05)。5)35%组的肝脏己糖激酶(HK)活性显著高于7%组(P<0.05),35%组的肝脏磷酸果糖激酶(PFK)活性显著高于其他各组(P<0.05),35%组的肝脏葡萄糖-6-磷酸脱氢酶(G6PDH)活性显著高于7%、14%和21%组(P<0.05),21%和28%组的肝脏果糖-1,6-二磷酸酶(FBPase)活性显著高于7%、14%和35%组(P<0.05)。6)35%组的肠道胃蛋白酶(PS)活性显著高于7%、14%、28%组(P<0.05),35%组的肠道脂肪酶(LPS)活性显著低于7%This experiment was conducted to explore the effects of dietary carbohydrate level on growth performance,body composition,plasma biochemical parameters and intestinal and liver enzyme activities of orange-spotted grouper(Epinephelus coioides).A total of 300 healthy orange-spotted grouper with initial weight of(102.80±1.02)g were randomly divided into 5 groups with 3 replicates per group and 20 fish per replicate.Fish in 5 groups were fed isonitrogenous and isoenergetic experimental diets which carbohydrate levels were 7% ,14% ,21% ,28% and 35% ,respectively.The experiment lasted for 810 weeks.The results showed as follows:1)the specific growth rate(SGR)and weight gain rate(WGR)of 28% group were significantly higher than those of other groups(P<0.05),the feed conversion ratio(FCR)of 28% group was significantly lower than that of 7% and 14% group(P<0.05),the protein efficiency ratio(PER),condition factor(CF)and hepatosomatic index(HSI)of 28% and 35% groups were significantly higher than those of 7% group(P<0.05),and the viscerosomatic index(VSI)of 35% group was significantly higher than that of 7% group(P<0.05).2)The apparent digestibility of dry matter of 7% group was significantly lower than that of 21% ,28% and 31% groups(P<0.05),and the apparent digestibilities of crude lipid and energy of 35% groups were significantly lower than those of 7% ,14% and 21% groups(P<0.05).3)The whole body crude protein content of 28% and 35% groups was significantly lower than that of 7% group(P<0.05),the whole body crude lipid content of 35% group was significantly higher than that of other groups(P<0.05),and the whole body ash content of 35% group was significantly lower than that of 7% ,14% and 28% groups(P<0.05).The muscle crude lipid content of 28% and 35% groups was significantly higher than that of 7% group(P<0.05),and the muscle ash content of 7% group was significantly higher than that of other groups(P<0.05).The liver glycogen content of 35% group was significantly higher than that of 7% ,14% and 21% groups(P<0.05),and

关 键 词：斜带石斑鱼 碳水化合物水平 生长性能 血浆生化指标 肝脏免疫酶 肠道消化酶 

分 类 号：S963[农业科学—水产养殖]