超声波辅助提取葛花总异黄酮及体外抗氧化活性研究  

Ultrasound-assisted Extraction of Total Isoflavones from Puerariae flos and Its Antioxidant Activity

作　　者：孙明哲[1] SUN Ming-zhe(Food and Biological Technology College,Changchun Vocational Institute of Technology,Changchun 130033,Jilin,China)

机构地区：[1]长春职业技术学院食品与生物技术分院

出　　处：《食品研究与开发》2020年第3期29-34,共6页Food Research and Development

基　　金：长春职业技术学院2019年度应用技术开发与研究项目（YY-2019C05）

摘　　要：以葛花为原料,总异黄酮得率为响应值,利用超声波辅助法,通过单因素和响应面法优化葛花总异黄酮提取工艺,并评价其体外抗氧化活性。结果显示,葛花总异黄酮超声波辅助提取的最佳条件为:超声时间40 min,超声功率260 W,乙醇浓度74%,超声温度76℃,固液比1∶16(g/mL),总异黄酮得率最高达到(12.35±0.37)mg/g。精制后的葛花总异黄酮对DPPH·、ABTS+·和O2^-·清除率可达到100%,同时具有一定的还原能力。葛花总异黄酮具有较强的体外抗氧化活性。The Puerariae flos were used as raw materials.The yield of total isoflavones was taken as the response value.Single factor and response surface method were used to optimize the extraction process of total isoflavones from Puerariae flos and evaluate its antioxidative activity in vitro.The results showed that the optimal conditions for extraction of total total isoflavones from Puerariae flos were as follows:ultrasonic time 40 min,ultrasonic power 260 W,ethanol volume fraction 74%,ultrasonic temperature 76℃,solid-liquid ratio 1∶16(g/mL),and the highest yield of total isoflavones was up to(12.35±0.37)mg/g.After purification,the total isoflavones in Puerariae flos had strong scavenging effects on DPPH·,ABTS+·and O2^-·,as the same time,the reducing power was also strong.The isoflavones from Puerariae flos showed strong antioxidant activity in vitro.

关 键 词：葛花 总异黄酮 超声波辅助 响应面法 抗氧化活性 

分 类 号：TQ464[化学工程—制药化工]