水稻光温敏核不育基因tms5与pms3的互作效应  

Interactive effects of the photoperiod-/thermo-sensitive genic male sterile genes tms5 and pms3 in rice

作　　者：王芳权[1,2] 范方军 夏士健[1] 宗寿余 郑天清[3] 王军[1,2] 李文奇[1,2] 许扬[1,2] 陈智慧[1,2] 蒋彦婕[1,2] 陶亚军 仲维功 杨杰 WANG Fang-Quan;FAN Fang-Jun;XIA Shi-Jian;ZONG Shou-Yu;ZHENG Tian-Qing;WANG Jun;LI Wen-Qi;XU Yang;CHEN Zhi-Hui;JIANG Yan-Jie;TAO Ya-Jun;ZHONG Wei-Gong;YANG Jie(Institute of Food Crops,Jiangsu Academy of Agricultural Sciences/Nanjing Branch of Chinese National Center for Rice Improvement/Jiangsu High Quality Rice R&D Center,Nanjing 210014,Jiangsu,China;Jiangsu Co-innovation Center for Modern Production Technology of Grain Crops,Yangzhou University,Yangzhou 225009,Jiangsu,China;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]江苏省农业科学院粮食作物研究所/国家水稻改良中心南京分中心/江苏省优质水稻工程技术研究中心,江苏南京210014 [2]扬州大学江苏省粮食作物现代产业技术协同创新中心,江苏扬州225009 [3]中国农业科学院作物科学研究所,北京100081

出　　处：《作物学报》2020年第3期317-329,共13页Acta Agronomica Sinica

基　　金：国家转基因生物新品种培育科技重大专项(2018ZX08001-02B);江苏省自然科学基金面上项目(BK20171326);江苏省现代农业重点研发项目(BE2018388,BE2017368)资助~~

摘　　要：tms5与pms3是水稻的光温敏核不育基因,其功能位点已经明确,然而它们在两系不育系中的效应尚不清楚。本研究针对tms5与pms3基因功能位点,分别设计了功能标记AS-TMS5和CAPS-PMS3。经鉴定发现,这2个功能标记能准确区分不育、可育性状对应的隐性纯合、杂合和显性纯合3种基因型。利用AS-TMS5和CAPS-PMS3对培矮64S/9311、广占63S/湘恢47和粤光S/宁恢108的F2群体单株的基因型及育性的关系分析发现,tms5基因是广占63S和粤光S控制光温敏不育性状的主效基因,而pms3基因在培矮64S和粤光S中并不能独立起作用,还需要与其他基因共同调控。进一步分析粤光S/宁恢108的F2:3群体基因型与育性的关系,发现在粤光S/宁恢108背景下,携带pms3基因的株系几乎都表现可育,而携带tms5基因的株系在较高气温条件下表现不育,但育性转换温度可能较高;而携带tms5与pms3基因的株系育性转换温度比仅携带tms5基因的株系低,这为聚合2个基因选育不育性状稳定的光温敏不育系提供了思路和方法。The tms5 and pms3 are two photoperiod-/thermo-sensitive genic male sterile genes,and their functional sites are clarified.However,the interactive effects of tms5 and pms3 in two-line sterile lines are still unclear.In this study,the functional markers AS-TMS5 and CAPS-PMS3 were designed,according to the functional sites of tms5 and pms3 respectively.The three genotypes of tms5 and pms3 were accurately distinguished by AS-TMS5 and CAPS-PMS3.The relationship of the phenotype and genotype in the F2 population of Pei’ai 64S/9311,Guangzhan 63S/Xianghui 47 and Yueguang S/Ninghui 108 were analyzed respectively.The tms5 was the major gene in Guangzhan 63S and Yueguang S,while pms3 was a non-independent gene in Pei’ai 64S and Yueguang S.By the phenotype and genotype analysis of the F2:3 population of Yueguang S/Ninghui 108,the plants carrying pms3 almost were fertile,while the plants carrying tms5 showed sterility,and had higher transition temperature.Furthermore,the sterility of the plants carrying tms5 and pms3 might have lower transition temperature than those carrying tms5.Pyramiding of tms5 and pms3 provides an efficient scheme to breed photoperiod-/thermo-sensitive genic male sterile lines,which have lower transition temperature and safer production.

关 键 词：tms5 pms3 功能标记 光敏不育 温敏不育 

分 类 号：S511[农业科学—作物学]