湖南省4株猪繁殖与呼吸综合征病毒全基因组序列测定与分析  

Determination and Analysis on the Complete Genomic Sequence of Four Strains of PRRS Virus in Hunan Province

作　　者：胡忆文 唐连飞 谭建锡 禹思宇 Hu Yiwen;Tang Lianfei;Tan Jianxi;Yu Siyu(Technology Center of Changsha Customs,Changsha,Hunan 410004,China)

机构地区：[1]长沙海关技术中心

出　　处：《中国动物检疫》2020年第2期66-70,共5页China Animal Health Inspection

基　　金：湖南省农业领域技术创新项目（2016NK2174）

摘　　要：为了解湖南省猪繁殖与呼吸综合征病毒(PRRSV)的遗传变异情况,对湖南省2016-2019年分离的4株PRRSV(Hunan/2016/075、Hunan/2017/085、Hunan/2018/123、Hunan/2019/055)进行全基因组测序,对其ORF5、NSP2基因以及全基因组核苷酸序列进行分析。结果显示,分离的4株病毒株均为美洲型,全基因组同源性为83.1%~98.7%,其中3株属于高致病型,1株为属于类NADC30型。Hunan/2016/075、Hunan/2017/085毒株全长序列与谱系5中亚系5.1的代表性毒株BJ-4亲缘关系相近,聚为一簇;Hunan/2018/123毒株与谱系8.7中的tjnh1501毒株聚为一簇;Hunan/2019/055毒株独立成一簇,与谱系1亲缘关系相近。本研究掌握了湖南省PRRSV的遗传变异情况,从而为制定有效的防控策略提供了依据。In order to recognize the genetic variation of porcine reproductive and respiratory syndrome virus(PRRSV)in Hunan Province,4 strains of PRRSV(Hunan/2016/075,Hunan/2017/085,Hunan/2018/123 and Hunan/2019/055)isolated from 2016 to 2019 in the province were sequenced,and their ORF5 and NSP2 genes and nucleotide sequences of their complete gene were analyzed.The results showed that all the four strains belonged into North American(NA)type,with the homology of complete genome of 83.1%to 98.7%,in which,3 strains were highly pathogenic and 1 strain was classified as NADC30-like strain.In view of the genetic relationship,the fulllength sequences of Hunan/2016/075 and Hunan/2017/085 were similar to BJ-4 strain,the representative one of 5.1 substrain of 5 family,which were clustered into one cluster;Hunan/2018/123 was clustered together with tjnh1501 strain of 8.7 family;and Hunan/2019/055 formed one cluster by itself,which was similar to 1 family concerning its genetic relationship.In short,the status of genetic variation of PRRSV in Hunan Province was identified,which would provide some references for developing effective control measures.

关 键 词：猪繁殖与呼吸综合征病毒 ORF5基因 NSP2基因 序列分析 

分 类 号：S852.65[农业科学—基础兽医学]