’禾荔’优质晚熟突变体’GLL-1’类黄酮糖基转移酶基因LcUFGT的克隆与表达分析  

Cloning and expression analysis of UDP glucose-flavonoid-3-o-glycosyltranferase gene LcU FGT from ’GLL-1’,a late-maturing mutant of ’Heli’

作　　者：张树伟[1] 彭宏祥[1] 潘介春[2] 李鸿莉[1] 秦献泉[1] 朱建华[1] 李冬波[1] 徐宁[1] 侯延杰[1] 邱宏业 李平 王金英 丁峰[1] ZHANG Shuwei;PENG Hongxiang;PAN Jiechun;LI Hongli;QIN Xianquan;ZHU Jianhua;LI Dongbo;XU Ning;HOU Yanjie;QIU Hongye;LI Ping;WANG Jinying;DING Feng(Horticultural Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China;College of Agricultural,Guangxi University,Nanning 530004,China;Agricultural Technology Extension Station of Madong Town,Guiping 537211,Guangxi,China)

机构地区：[1]广西壮族自治区农业科学院,园艺研究所,南宁530007 [2]广西大学农学院,南宁530004 [3]广西桂平市麻垌镇农业技术推广站,广西桂平537211

出　　处：《广西植物》2020年第1期128-135,共8页Guihaia

基　　金：国家重点研发计划项目(2019YFD1000904);国家自然科学基金(31760564);“广西八桂青年学者”专项项目(BGQN201979-1);国家荔枝龙眼产业技术体系项目(CARS-33-03);广西自然科学基金(2017GXNSFAA198350,2016GXNSFBA380036,2018GXNSFAA050089);广西科技重大专项(桂科AA17204026,桂科AA17204097);广西荔枝龙眼创新团队建设专项(nycytxgxcxtd-01);亚热带农业生物资源保护与利用国家重点实验室开放课题(SKLCUSA-b201811);广西农业科学院基本科研业务专项(桂农科2018YM21)~~

摘　　要：’GLL-1’为’禾荔’中选育出的一个优质晚熟芽变新种质。为探讨’GLL-1’果实成熟期延迟的遗传基础,该研究以’禾荔’和’GLL-1’为实验材料,克隆花色素苷合成途径结构基因LcUFGT,并对其进行生物信息学预测及分析,同时通过qRT-PCR对LcUFGT基因在果实发育不同阶段的表达进行研究,分析LcUFGT的表达对突变体果实发育速度的影响。结果表明:(1)LcUFGT基因ORF长1359 bp,编码453个氨基酸,推测蛋白质分子量约为50.16 kD。(2)序列比对发现所编码蛋白与’禾荔’等荔枝品种高度保守,在蛋白的C端具有PSPG盒。(3)在果实发育成熟进程中,’禾荔’和’GLL-1’果皮逐渐退绿转红,两者LcUFGT基因的表达量都呈先上升后下降的表达趋势,然而,’禾荔’LcUFGT基因的表达量在花后56 d显著增加,突变体’GLL-1’LcUFGT基因的表达量在花后67 d显著增加,LcUFGT基因在突变体’GLL-1’中显著上升表达的时间比’禾荔’延迟,且与果实发育延迟基本一致。以上结果表明,LcUFGT基因在荔枝果皮着色过程中发挥重要作用,是果实着色的关键基因之一,突变体’GLL-1’中的延迟表达是引起突变体晚熟的原因之一。’GLL-1’is an excellent late-maturing bud sport germplasm,which was selected from’Heli’.To reveal the genetic basis of late-maturing,in this study,’Heli’and’GLL-1’were used as the materials.The LcUFGT which is a structural gene of anthocyanin synthesis pathway was cloned and analyzed by bioinformatic methods.The corelation between LcUFGT and the character of late-maturing in’GLL-1’and the expression of LcUFGT during the development of litchi fruit were analyzed by q RT-PCR.The results were as follows:(1)ORF of LcUFGT gene was 1359 bp,which encoded 453 amino acids with the molecular weight of 50.16 k D.(2)LcUFGT gene was conservative in’GLL-1’and other litchi cultivars,and a PSPG box existed in the C-terminal.(3)With the development of litchi fruit,the color of pericarp began to turn from green to red;the expression of LcUFGT was increased and then decreased in both’GLL-1’and’Heli’;the expression of LcUFGT was obviously increased at 56 d and 67 d after flowering of’Heli’and’GLL-1’,respectively;the expression of LcUFGT had a delayed increase in’GLL-1’than that of’Heli’,which was consistent with the late-maturing of fruits.It is supposed that LcUFGT plays an important role in color changes of pericarp,and it is one of the key genes to regulate coloration of pericarp.At the same time,the delay-expression of LcUFGT maybe the main reason of late-maturing of’GLL-1’.

关 键 词：晚熟突变体 LcUFGT基因 果实发育 花色素苷合成 表达分析 

分 类 号：Q943[生物学—植物学]