凋亡细胞调控巨噬细胞表达IL-12家族细胞因子的水平  

Apoptotic cells regulate the level of IL-12 family in macrophages

作　　者：刘冰慧[1] 龚志平[1] 梅艳芳[1] 杨涛[1] 冯军[1] 钱雪松 LIU Bing-hui;GONG Zhi-ping;MEI Yan-fang;YANG Tao;FENG Jun;QIAN Xue-song(Department of Clinical Laboratory,Chengde Center Hospital,Hebei Province,Chengde 067000,China;Department of Immunology,the School of Basic Medicine Sciences,Hebei Medical University,Shijiazhuang 050017,China)

机构地区：[1]河北省承德市中心医院检验科,河北承德067000 [2]河北医科大学基础医学院免疫学教研室,河北石家庄050017

出　　处：《河北医科大学学报》2020年第1期89-93,共5页Journal of Hebei Medical University

基　　金：河北省医学科学研究重点课题(20160300)

摘　　要：目的观察凋亡细胞对巨噬细胞中白细胞介素12(interleukin-12,IL-12)家族细胞因子表达的影响。方法将小鼠巨噬细胞系RAW264.7(RAW细胞)和小鼠腹腔巨噬细胞分别分为空白对照组、脂多糖(lipopolysaccharides,LPS)刺激组(LPS组)、凋亡细胞刺激组(apo组)、脂多糖+凋亡细胞刺激组(LPS+apo组),实时定量PCR检测各组细胞刺激前后mIL-12p35、mIL-12p40、mIL-23p19水平。采用酶联免疫吸附测定方法检测各组激活的RAW细胞培养液中IL-10、前列腺素E 2(prostaglandin E 2,PGE 2)和转化生长因子β(transforming growth factor-β,TGF-β)的含量。结果RAW细胞和小鼠腹腔巨噬细胞LPS组IL-12p35、IL-12p40、IL-23p19 mRNA表达量高于空白对照组和apo组,LPS+apo组IL-12p35、IL-12p40、IL-23p19 mRNA表达量高于空白对照组和apo组,低于LPS组(P<0.05)。LPS组和LPS+apo组IL-10、PGE 2、TGF-β浓度均高于对照组,LPS+apo组TGF-β浓度高于对照组和LPS组(P<0.05);LPS组和LPS+apo组IL-10、PGE 2浓度差异无统计学意义(P>0.05)。结论凋亡细胞抑制活化巨噬细胞IL-12p35、IL-12p40、IL-23p19 mRNA的表达。凋亡细胞对IL-10和PGE 2分泌无影响,但促进TGF-β的分泌。Objective To investigate the effect of apoptotic cells on expression of interleukin-12(IL-12)family cytokines in macrophages.Methods RAW264.7(RAW cells)from the mouse macrophage cell lineage and mouse peritoneal macrophages were divided into 4 groups respectively:blank control group,lipopolysaccharide(LPS)stimulation group,apoptotic(apo)cell stimulation group,LPS+apo cell stimulation group,the levels of mIL-12p35,mIL-12p40 and mIL-23p19 in macrophage cell of each group before and after the stimulation were detected by real-time quantitative polymease chain reaction(PCR).The contents of IL-10,prostaglandin E 2(PGE 2)and transforming growth factor-β(TGF-β)in RAW cell supernatant were detected by enzyme linked immunosorbent assay(ELISA).Results The mRNA expression level of IL-12p35,IL-12p40 and IL-23p19 in RAW cells or mouse peritoneal macrophages were higher in LPS group than in blank control group and apo group.The mRNA level of IL-12p35,IL-12p40 and IL-23p19 in the LPS+apo group were higher than those in the blank control group and in the apo group,and lower than those in the LPS group(P<0.05).The concentrations of IL-10,PGE 2 and TGF-βin the LPS group or LPS+apo group were all higher than those in the control group,and the concentrations of TGF-βin LPS+apo group were significantly higher than those in the LPS group(P<0.05),while the differences of IL-10 or PGE 2 concentrations in the LPS group and LPS+apo group were not statistically significant(P>0.05).Conclusion Apoptotic cells inhibit the expression of IL-12p35,IL-12p40 and IL-23p19 mRNA in activated macrophages.Apoptotic cells have no effect on the secretion of IL-10 and PGE 2,but can promote the secretion of TGF-βin macrophages.

关 键 词：巨噬细胞 凋亡细胞 白细胞介素12 

分 类 号：R446.63[医药卫生—诊断学]