含Hp特异性抗体羊奶2种检测方法的比较及初步临床效果  

Comparison of the two methods in the detection of goat-milk containing anti-Helicobacter pylori specific antibody and preliminary clinical effect

作　　者：颜晰[1] 樊建平 王苋[3] 冯剑飞 赵连梅[4] 单保恩[4] YAN Xi;FAN Jian-ping;WANG Xian;FENG Jian-fei;ZHAO Lian-mei;SHAN Bao-en(Department of Clinical Laboratory,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,China;Department of Digestive Endoscopy,Jingxing County Hospital,Hebei Province,Jingxing 050300,China;Microbiological Inspection Institute,Shijiazhuang Center for Disease Control and Prevention,Hebei Province,Shijiazhuang 050011,China;Research Center,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,China)

机构地区：[1]河北医科大学第四医院检验科,河北石家庄050011 [2]河北省井陉县医院消化内镜科,河北井陉050300 [3]河北省石家庄市疾病预防控制中心微生物检验所,河北石家庄050011 [4]河北医科大学第四医院科研中心,河北石家庄050011

出　　处：《河北医科大学学报》2020年第1期94-98,共5页Journal of Hebei Medical University

基　　金：国家自然科学基金面上项目(81673642);河北省卫生健康领域技术研究暨成果转化重点项目(zh2018008)

摘　　要：目的比较含抗Hp特异性抗体羊奶的2种检测方法,并初步观察抗体羊奶的临床效果。方法用国际标准菌株ATCC26695及ATCC26695、SS1混合菌液制备2种含抗Hp特异性抗体的羊奶,分别用双相免疫扩散试验及酶联免疫吸附测定(enzyme linked immunosorbent assay,ELISA)法检测羊奶中抗Hp特异性抗体含量,并比较二者检测效果;30名受试者按照随机抽样法分为试验组和对照组,试验组每天饮用抗体羊奶,对照组饮用普通羊奶,连续饮用30 d,用14 C尿素呼气试验(14 C urea breath test,14 C-UBT)评估效果。结果2种方法检测结果均证实了抗体羊奶中抗Hp特异性抗体的存在,ELISA结果显示,2种抗体羊奶中抗Hp特异性抗体含量高于对照孔,差异有统计学意义(P<0.05),且随着稀释倍数增大,抗体含量逐渐降低;2种抗体羊奶间抗体含量差异无统计学意义(P>0.05)。初步临床试验证明试验组经服用抗体羊奶后有效率为66.6%,与服用前比较差异有统计学意义(P<0.05)。结论2种检测方法均可较好地反映抗体羊奶中抗Hp特异性抗体存在,ELISA法简便、快速,能够更为精确地定量检测抗体含量,所获得的结果更为可靠。临床试验表明抗体羊奶能够有效降低患者14 C-UBT数值,具有良好的临床应用前景。Objective To compare two detection methods of goat-milk containing anti-Helicobacter pylori specific antibody,and to observe the clinical effect of antibody goat-milk.Methods Goat-milk containing antibodies against Helicobacter pylori was prepared by immunizing dairy goat with international standard strains(ATCC26695,ATCC26695 and SS1 mixture).The valence of antibody against Helicobacter pylori in goat-milk was detected by double immunodiffusion test and enzyme linked immunosorbent assay(ELISA),and then compare the two methods.30 subjects were randomly assigned to two groups.The test group were treated with goat-milk containing Helicobacter pylori specific antibody,and control group with common goat-milk,drink for 30 days continuously.The effect was assessed by the 14 C urea breath test(14 C-UBT).Results The results of the two methods all confirmed the existence of anti-Helicobacter pylori specific antibodies in goat milk,ELISA test show that the content of antibodies in the two goat-milk was higher than that in the control,the difference was statistically significant(P<0.05),and with the increase of dilution ratio,the antibody content decreased gradually.There was no significant difference in antibody content between the two kinds of goat milk(P>0.05).Preliminary clinical trials have shown that the effective rate of the experimental group was 66.6%after taking the antibody goat-milk,and the difference was statistically significant(P<0.05)compared with the control group.Conclusion Both of the two detection methods can better prove the existence of anti-Helicobacter pylori specific antibodies in goat-milk.Methed of ELISA is simple and fast,which can more accurate quantitatively detect antibody content.And the results are more reliable.Clinical trials have shown that goat-milk containing Helicobacter pylori specific antibody can effectively reduce the value of 14 C-UBT in patients,it has a good prospect of clinical application.

关 键 词：幽门螺杆菌 抗幽门螺杆菌特异性抗体 抗体羊奶 

分 类 号：R377[医药卫生—病原生物学] R186.4[医药卫生—基础医学]