自贡地区HBV基因BCP区1762/1764及前C区1896位点突变与HBV相关性肝癌的相关性研究  

Correlation between HBV gene BCP region 1762/1764 and pre-C region 1896 mutation and HBV-associated liver cancer in Zigong,West China

作　　者：邱顺华[1] 张德文[1] 刘勇[2] QIU Shunhua;ZHANG Dewen;LIU Yong(Department of Clinical Laboratory,Zigong third people􀆳s hospital,Zigong,Sichuan,China,643020;Department of Cardiology,Zigong third people􀆳s hospital,Zigong,Sichuan,China,643020)

机构地区：[1]自贡市第三人民医院检验科,四川自贡643020 [2]自贡市第三人民医院心内科,四川自贡643020

出　　处：《分子诊断与治疗杂志》2020年第1期16-21,共6页Journal of Molecular Diagnostics and Therapy

基　　金：自贡市卫生和计划生育委员会重点立项项目(自卫办发[2015]70号08);自贡市科技局项目(自科发[2016]39号ZC201609)

摘　　要：目的通过分析自贡地区乙肝患者乙型肝炎病毒HBV基因BCP区1762/1764及前C区1896位点的突变情况,探讨其与HBV相关性肝癌的关系。方法收集2015年9月至2018年6月141例经本院确诊的乙肝性相关疾病患者血清标本(HBV DNA≥103IU/mL):慢性乙肝(CHB组)50例、肝硬化(LC组)45例、肝癌(HCC组)46例,并收集临床相关资料。采用荧光定量PCR法检测患者的HBV DNA水平,多通道荧光PCR法检测HBV基因型,然后采用ARMS-PCR法检测HBV基因BCP区1762/1764及前C区1896突变情况。采用SPSS17.0软件对数据进行统计学分析。结果HCC组和CHB组在e抗原阳性率、HBV DNA水平及HBV DNA>105IU/mL方面对比发现差异均具有统计学意义(P<0.05);HCC组和LC组在e抗原阳性率方面比较发现差异无统计学意义(P>0.05),而HBV DNA水平方面差异具有统计学意义(P<0.05)。男性、女性乙肝患者HBV基因BCP区1762/1764位点突变率分别为67.0%、57.1%(P>0.05),前C区1896位点突变率分别为61.6%、66.7%(P>0.05)。HCC组患者、LC组患者、CHB组患者的HBV基因BCP区1762/1764突变率分别为91.3%、84.4%、22%,HCC组与CHB组比较差异具有统计学意义(P<0.05),而HCC组与LC组比较差异无统计学意义(P>0.05);HBV基因前C区1896突变率分别为84.8%、62.2%、42.0%,HCC组与CHB组、LC组比较差异均具有统计学意义(P<0.05)。HCC组患者的HBV基因前C区1896和BCP区1762/1764位点同时突变率为78.3%,要高于LC组和CHB组,差异均具有统计学意义(P<0.05)。HBV基因型方面比较,无论是基因B型还是C型的乙肝相关患者,疾病进程较重(HCC组、LC组)的受试者携带BCP区1762/1764突变型或前C区1896突变型的比例都要高于慢性乙肝患者(CHB组)。结论自贡地区HBV基因BCP区1762/1764和前C区1896突变率高,无性别和基因型差异,其中HBV基因BCP区1762/1764位点和前C区1896位点联合突变与HBV相关性HCC发生可能存在一定关系。Objective To analyze the mutations of HBV gene BCP region 1762/1764 and pre⁃C region 1896 in patients with hepatitis B in Zigong area,and to explore its relationship with HBV⁃related liver cancer.Methods 141 cases of hepatitis B related diseases with HBV DNA level≥103 IU/mL from September 2015 to June 2018 were collected:50 cases of chronic hepatitis B(CHB),45 cases of liver cirrhosis(LC),and 46 cases of HBV HCC.HBV DNA level was detected by fluorescence quantitative PCR,HBV genotype was detected by multi⁃channel fluorescence PCR,and HBV gene mutations in BCP 1762/1764 and PC 1896 were detected by ARMS⁃PCR.Software SPSS 17.0 was used for statistical analysis.Results There were significant differences in e⁃antigen(HBeAg)positive rate,HBV DNA level and HBV DNA>105 IU/mL percent between HCC and CHB group(P<0.05).There was no significant difference in e⁃antigen positive rate between HCC g and LC group(P>0.05),while the difference in HBV DNA level and DNA>105 IU/mL percent was statistically significant(P<0.05).The mutation rates of male and female hepatitis B patients in the BCP region of the HBV gene at 1762/1764 were 67.0%and 57.1%(P>0.05),and the mutation rates at the 1896 site in the former C region were 61.6%and 66.7%(P>0.05).The 1762/1764 mutation rates in the BCP region of the HBV gene of patients in the HCC group,LC group,and CHB group were 91.3%,84.4%,and 22.0%,respectively.The difference between the HCC group and the CHB group was statistically significant(P<0.05).And there was no statistically significant difference between the HCC group and the LC group(P>0.05).The 1896 mutation rates in the pre⁃C region of the HBV gene were 84.8%,62.2%,and 42.0%,respectively.The PC 1896 mutation rate of HCC was markedly different with that of CHB and LC(P<0.05).In HCC group,78.3%of patients had both HBV gene PC 1896 mutations and BCP 1762/1764 mutations,which were significantly higher than 48.9%and 0.00%in CHB group and LC group(P<0.05).In chronic HBV infection patients with genotype B and genotype C,the m

关 键 词：乙型肝炎病毒 BCP区1762/1764 前C区1896 乙肝相关性肝癌 

分 类 号：R512.62[医药卫生—内科学] R735.7[医药卫生—临床医学]