枸杞内生真菌对胶孢炭疽菌Colletotrichum gloeosporioides的拮抗作用及生防潜力  

Antagonism and control potential of endophytic fungi from Lycium barbarum against Colletotrichum gloeosporioides

作　　者：胡丽杰 闫思远 李嘉泓 杜娟 顾沛雯[1] HU Lijie;YAN Siyuan;LI Jiahong;DU Juan;GU Peiwen(School of Agriculture,Ningxia University,Yinchuan 750021,China)

机构地区：[1]宁夏大学农学院

出　　处：《植物保护》2020年第1期125-133,共9页Plant Protection

基　　金：国家自然科学基金(31460484)

摘　　要：为了明确枸杞内生真菌对胶孢炭疽菌的抑制作用,通过室内抑菌试验和离体试验测定了内生真菌对胶孢炭疽菌的抑制作用及对枸杞炭疽病的防治效果。结果表明,镰刀菌属Fusarium菌株NQ8GII4、NQ8GII7、NQ7GII4和篮状菌属Talaromyces菌株NQ6GIII11对胶孢炭疽菌有明显的拮抗作用。平板对峙试验显示,上述4株枸杞内生真菌具有较强的营养和空间竞争能力,菌株NQ8GII4对病原菌的抑制率高达93.43%。显微观察发现,枸杞内生真菌能缠绕并穿透胶孢炭疽菌菌丝,使胶孢炭疽菌菌丝膨胀崩解。菌株NQ7GII4的培养滤液在浓度为15%时对病原菌菌丝的抑制率达到70.75%,菌株NQ8GII7产生的挥发性物质对胶孢炭疽菌抑制率高达83.44%。离体生防试验结果表明,用拮抗性内生真菌预先占位接种枸杞嫩果及叶片,能有效抑制枸杞炭疽病菌的侵入和病斑的扩展,菌株NQ8GII410%培养滤液对枸杞炭疽病的防治效果最强,防效达到了92.54%和95.57%,说明枸杞内生真菌具有很好的生防潜力和应用前景。In order to explore the inhibitory effect of the endophytic fungi from Lycium barbarum against Colletotrichum gloeosporioides,their inhibitory effects and biocontrol potential against the anthracnose were determined by using indoor bacteriostatic test and in vitro test.The results showed that Fusarium strains NQ8GII4,NQ8GII7,NQ7GII4 and Talaromyces strain NQ6GIII11 had obvious antagonistic effects on C.gloeosporioides.The plate confrontation test showed that the above four endophytic fungi had strong nutrition and space competitiveness,and the inhibition rate of strain NQ8GII4 against the pathogenic bacteria reached 93.43%.According to microscopic observation,endophytic fungi from L.barbarum could entangle and penetrate the hyphae of C.gloeosporioides,leading to disintegration of the hyphae of C.gloeosporioides.When the concentration of NQ7GII4 culture filtrate was 15%,the inhibition rate of mycelium was 70.75%,while the inhibition rate of volatile substances produced by strain NQ8GII7 against C.gloeosporioides was as high as 83.44%.The results of in vitro biocontrol experiments showed that vaccination of antagonistic endophytic fungi in tender fruits and leaves of L.barbarum could effectively inhibit the invasion of L.barbarum anthracnose and the expansion of lesions.The culture filtrate of strain NQ8GII410%had the strongest control effect on L.barbarum anthracnose,and the control effect reached to 92.54%and 95.57%,indicating that L.barbarum endophytic fungi had good biocontrol potential and application prospects.

关 键 词：枸杞内生真菌 胶孢炭疽菌 拮抗作用 生物防治 

分 类 号：S435.671[农业科学—农业昆虫与害虫防治]