DMSO联合神经营养因子BDNF诱导BMSCs向神经样细胞分化  

Differentiation of BMSCs into Neuron-Like Cells Induced by DMSO Combined with Neurotrophic Factor BDNF

作　　者：倪爽 苗泽远 王家昕 王晓霞 李洁 王子钰 魏会平[2] 董明纲 苏立宁[2] NI Shuang;MIAO Ze-yuan;WANG Jia-xin;WANG Xiao-xia;LI Jie;WANG Zi-yu;WEI Hui-ping;DONG Ming-gang;SU Li-ning(Anesthesia Major,Basic Medical College of Hebei North University,Zhangjiakou,075000,China;Clinical Speciality,Basic Medical College of Hebei North University,Zhangjiakou,075000,China;Stomatology,Basic Medical College of Hebei North University,Zhangjiakou,075000,China;Department of Biology,Basic Medical College of Hebei North University,Zhangjiakou,075000,China;Editorial Department of Journal of Hebei North University,Zhangjiakou,075000,China)

机构地区：[1]河北北方学院基础医学院,中国张家口075000 [2]河北北方学院基础医学院生物教研室,中国张家口075000 [3]河北北方学院学报编辑部,中国张家口075000

出　　处：《神经药理学报》2019年第5期5-9,16,共6页Acta Neuropharmacologica

基　　金：2018年国家级大学生创新创业训练计划项目(No.201810092001);河北北方学院青年基金项目(No.QN2018022)

摘　　要：目的:探讨脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)联合二甲基亚砜(dimethyl sulfoxide,DMSO)诱导骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)向神经样细胞分化的效果,初步检测miRNA-26a在神经细胞分化过程中的表达量变化。方法:从大鼠股骨抽取骨髓分离BMSCs,DMSO对其预诱导,随后用含BDNF的神经细胞培养基培养BMSCs,诱导其分化为神经细胞。免疫组化鉴定分化神经元样细胞特异标记物:神经元特异烯醇酶(neuron specific enolase,NSE)。实时定量PCR法检测神经分化前后酪氨酸磷酸酶蛋白(tyrosine phosphatase protein,PTEN)基因/mTOR和miRNA-26a的表达变化。结果:100 ng·mL^-1 BDNF联合3%DMSO可诱导BMSCs向神经元样细胞分化,分化后神经细胞标志物NSE蛋白表达呈阳性,且神经分化过程中miRNA-26a、PTEN/mTOR的含量明显上调。结论:3%DMSO和100 ng·mL^-1 BDNF的混合诱导剂可诱导大鼠BMSCs向神经元样细胞分化,而miRNA-26a可能通过PTEN/mTOR调节神经分化。Objective:To investigate the effect of BDNF combined with DMSO in inducing BMSCs differentiation into neuron-like cells,and to detect the expression of miRNA-26 a in the process of neuron differentiation. Methods:BMSCs were isolated from rat femoral bone marrow and preinduced by DMSO. BMSCs were then cultured in a neuronal medium containing BDNF and differentiated into neurons. Differentiated neuron-like cell-specific marker neuron-specific enolase(NSE)was identified using immunohistochemical. Real-time quantitative PCR was used to detect the expression of PTEN/mTOR and miRNA-26 a before and after neurodifferentiation. Results:BDNF combined with DMSO had significant effect in inducing BMSCs differentiation into neuron-like cells,and the contents of miRNA-26 a and PTEN/mTOR were significantly increased during the process of neurodifferentiation. Conclusion:Mixed inducers of DMSO and BDNF can induce BMSCs differentiation into neuron-like cells in rats,and miRNA-26 a may regulate neuronal differentiation through PTEN/mTOR.

关 键 词：脑源性神经营养因子 二甲基亚砜 骨髓间充质干细胞 神经分化 miRNA-26a 

分 类 号：R965[医药卫生—药理学]