PPAR-γ激动剂对炎症状态下人系膜细胞LOX-1表达的影响  

Effect of PPAR-γ agonist on the expression of LOX-1 in human mesangial cells under inflammatory conditions

作　　者：刘华[1] 李银平[1] 吴雷云[1] 林娜[1] 付文静[1] 李文[1] 董星彤[1] 贾林沛 贾强[1] 邓英辉[1] Liu Hua;Li Yinping;Wu Leiyun;Lin Na;Fu Wenjing;Li Wen;Dong Xingtong;Jia Linpei;Jia Qiang;Deng Yinghui(Department of Nephrology,Xuanwu Hospital,Capital Medical University,Beijing 100053,China)

机构地区：[1]首都医科大学宣武医院肾内科

出　　处：《中华肾病研究电子杂志》2019年第6期263-268,共6页Chinese Journal of Kidney Disease Investigation（Electronic Edition）

摘　　要：目的研究过氧化物酶体增殖体激活受体γ(PPAR-γ)激动剂15-脱氧前列腺素J 2(15d-PGJ 2)对炎症因子白细胞介素1β(IL-1β)作用下人肾小球系膜细胞(HMC)氧化低密度脂蛋白(Ox-LDL)植物血凝素样受体1(LOX-1)表达的影响。方法激光共聚焦显微镜观察IL-1β对HMC脂质摄取的影响;实时定量PCR和Western印迹方法检测不同剂量的PPAR-γ激动剂15d-PGJ 2对IL-1β诱导的LOX-1表达的影响。结果激光共聚焦显微镜检查发现5 ng/ml的IL-1β促进HMC摄取荧光标记的Ox-LDL;IL-1β能够促进HMC的LOX-1 mRNA和蛋白表达,15d-PGJ 2呈剂量依赖性抑制IL-1β诱导的LOX-1表达。与单纯IL-1β(5 ng/ml)刺激组相比,15d-PGJ 2浓度2.5、5、10μmol/L处理组细胞LOX-1 mRNA表达分别下调为73.13%、66.54%、35.23%;15d-PGJ 2浓度5、10μmol/L处理组细胞LOX-1蛋白表达分别下调为82.39%、63.17%。结论炎症因子IL-1β促进HMC摄取Ox-LDL;PPAR-γ激动剂15d-PGJ 2剂量依赖性抑制IL-1β诱导的LOX-1 mRNA和蛋白表达,对炎症因子导致的系膜细胞脂质失衡可能具有保护作用。Objective To investigate the effect of peroxisome proliferator-activated receptorγ(PPAR-γ)agonist 15-deoxyprostaglandin J 2(15d-PGJ 2)on the expression of lectin-like oxidized low-density lipoprotein(Ox-LDL)receptor 1(LOX-1)in human glomerular mesangial cells(HMC)treated with interleukin 1β(IL-1β).Methods Laser confocal microscopy was used to observe the effect of IL-1βon lipid uptake by HMC.Real-time quantitative PCR and Western blotting were applied to study the effects of different doses of PPAR-γagonist 15d-PGJ 2 on IL-1β-induced LOX-1 expression.Results Laser confocal microscopy revealed that IL-1β(5 ng/ml)promoted HMC uptake of Ox-LDL.IL-1βpromoted the mRNA and protein expression of LOX-1 in HMC,while 15d-PGJ 2 dose-dependently inhibited IL-1β-induced LOX-1 expression.Compared with the IL-1β(5ng/ml)group,the treatment group showed that 15d-PGJ 2 in concentrations of 2.5μmol/L,5μmol/L,and 10μmol/L down-regulated the mRNA expression of LOX-1 to 73.13%,66.54%,and 35.23%,respectively,while 15d-PGJ 2 in concentrations of 5μmol/L and 10μmol/L down-regulated the protein expression of LOX-1 to 82.39%and 63.17%,respectively.Conclusion Inflammatory factor IL-1βpromoted the uptake of Ox-LDL and expression of LOX-1 in HMC.PPAR-γagonist 15d-PGJ 2 dose-dependently inhibited the mRNA and protein expression of LOX-1 induced by IL-1β,which may have a protective role in the lipid imbalance of HMC caused by inflammatory factors.

关 键 词：过氧化物酶体增殖体激活受体γ激动剂 白细胞介素1β 氧化低密度脂蛋白植物血凝素样受体1 

分 类 号：R692[医药卫生—泌尿科学]