HPLC同时测定肺宁制剂中4种成分的含量  

Simultaneous Determination of Four Constituents in Feining Preparation by HPLC

作　　者：程世云 张亚中 韩玲玲 王浩 王满媛 周志凌 CHENG Shiyun;ZHANG Yazhong;HAN Lingling;WANG Hao;WANG Manyuan;ZHOU Zhiling(Anhui Provincial Institute for Food and Drug Control,Hefei 230051,China)

机构地区：[1]安徽省食品药品检验研究院

出　　处：《中国现代应用药学》2019年第22期2814-2817,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：2018年国家药品抽验计划项目(食药监药化监[2017]131号)

摘　　要：目的建立HPLC同时测定肺宁颗粒、肺宁胶囊和肺宁片中新绿原酸、绿原酸、隐绿原酸和金丝桃苷4种成分的含量。方法采用Waters XBridge TM C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.4%磷酸水溶液,梯度洗脱,流速1.0 mL·min^-1;检测波长327,353 nm;柱温30℃。结果4种成分在各自范围内线性关系良好(r>0.9990),平均加样回收率96.1%~104.7%,RSD为0.4%~1.6%。结论该方法准确、可靠,重复性好,可用于肺宁制剂的质量控制。OBJECTIVE To establish an HPLC method for the simultaneous determination of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid and hyperin in Feining granules,Feining capsules and Feining tablets.METHODS Preparation was performed on a 30℃Waters XBridge TM C18 column(4.6 mm×250 mm,5μm),with the mobile phase comprising of acetonitrile-0.4%phosphoric acid flowing at 1.0 m L·min^-1 in a gradient elution manner,and the detection wavelengths were set at 327,353 nm.RESULTS Four constituents showed good linear relationships with their own ranges(r>0.9990),while the average recoveries were 96.1%-104.7%with the RSDs of 0.4%-1.6%.CONCLUSION This accurate,reliable and reproducible method can be used for the quality control of Feining preparation.

关 键 词：肺宁颗粒 肺宁胶囊 肺宁片 新绿原酸 绿原酸 隐绿原酸 金丝桃苷 高效液相色谱法 

分 类 号：R917.101[医药卫生—药物分析学]