HPLC-荧光法测定酒女贞子中羟基酪醇、红景天苷和酪醇  

Determination of Hydroxytyrosol, Salidroside and Tyrosol in Wine-fried Ligustri Lucidi Fructus by HPLC with Fluorescence Detection

作　　者：廖晓燕[1] 洪圆 姜雯文 梁淑彩[1] LIAO Xiaoyan;HONG Yuan;JIANG Wenwen;LIANG Shucai(Wuhan University School of Pharmaceutical Sciences,Wuhan 430071,China)

机构地区：[1]武汉大学药学院

出　　处：《中国现代应用药学》2019年第24期3064-3067,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：武汉大学教学改革研究项目(JG201670,2018JG031)

摘　　要：目的建立一种简单、高灵敏度,可同时测定酒女贞子中羟基酪醇、红景天苷和酪醇含量的HPLC-荧光法。方法采用AmethystC18-P柱(250mm×4.6mm,5μm),以水-乙腈(92∶8)为流动相等度洗脱,激发波长为280nm,发射波长为310 nm,柱温35℃。结果羟基酪醇、红景天苷和酪醇在一定浓度范围内线性关系良好,检测限分别可达23.0,10.5,9.82 ng·mL^-1。酒女贞子中羟基酪醇、红景天苷和酪醇的含量范围分别为642.9~867.2μg·g^-1,1932.3~15357.5μg·g^-1,239.1~638.1μg·g^-1。结论所建立的HPLC-荧光法灵敏度高,选择性好、准确度高,可用于酒女贞子中羟基酪醇、红景天苷和酪醇的含量测定。OBJECTIVE To develop a simple and sensitive HPLC with fluorescence detection method for simultaneous determination of hydroxytyrosol, salidroside and tyrosol in wine-fried Ligustri Lucidi Fructus. METHODS The analysis was performed on a Amethyst C18-P column(250 mm×4.6 mm, 5 μm) with isometric elution using water and acetonitrile(92∶8). The detection wavelengths were at 280 nm and 310 nm for excitation and emission. The column temperature was 35 ℃. RESULTS The good linearity was in the certain concentration range, and the limit of quantitation of hydroxytyrosol, salidroside and tyrosol were 23.0, 10.5, 9.82 ng·mL^-1, respectively. The range of content of hydroxytyrosol, salidroside and tyrosol in wine-fried Ligustri Lucidi Fructus were 642.9-867.2 μg·g^-1, 1 932.3-15 357.5 μg·g^-1, 239.1-638.1 μg·g^-1, respectively. CONCLUSION The developed HPLC-Fluorescence detection method is sensitive, selective and accurate for simultaneous determination of hydroxytyrosol, salidroside and tyrosol in wine-fried Ligustri Lucidi Fructus.

关 键 词：高效液相色谱法 荧光 羟基酪醇 红景天苷 酪醇 酒女贞子 

分 类 号：R917.101[医药卫生—药物分析学]