无细胞合成生物学：革新生物医药产业的新策略  

Cell-free synthetic biology: an emerging strategy torevolutionize the biomedical industry

作　　者：刘莹莹 卜宁[1] 卢元[2] Yingying Liu;Ning Bu;Yuan Lu(College of Life Sciences,Shenyang Normal University,Shenyang 110034,Liaoning,China;Department of Chemical Engineering,Tsinghua University,Beijing 100084,China)

机构地区：[1]沈阳师范大学生命科学学院,辽宁沈阳110034 [2]清华大学化学工程系,北京100084

出　　处：《生物工程学报》2019年第12期2269-2283,共15页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(Nos.21706144,21878173);北京市自然科学基金(No.2192023)资助~~

摘　　要：无细胞合成生物系统,能够在体外完成生命转录翻译过程,因体系灵活开放、便于控制、表达周期短、高耐受性等特点,可表达细胞系统难以表达的蛋白质。随着无细胞生物传感和体系冻干技术的不断发展,其在医药健康领域的应用不断拓展。本文综述了无细胞合成生物学在按需生物医药合成和便携式医疗检测等医药健康领域的研究进展,该体系的进一步发展有潜力实现更复杂后修饰蛋白质药物的合成、可丰富无细胞生物传感器类型并提高其灵敏性。无细胞合成生物学作为新兴工程策略,未来必将更好地应用于高通量医药蛋白质筛选、新型病原体的检测等医药健康领域。Cell-free synthetic biology system can perform biological transcription and translation process in vitro.Because of its advanced features,such as flexible openness,easy control,short expression time and high tolerance to cytotoxicity,this systemhas been successfully used to synthesize proteins that are difficult to express in cells.With the continuous development of cell-free biosensing technology and the lyophilization technology,its applications have widely expanded into many biomedical fields.This review discusses the current research progress of cell-free synthetic biology system in on-demand biopharmaceutical synthesis,portable diagnostics,and others.Further development of the system can lead to even more complicated synthesis of therapeutic proteins with post-translational modifications and evolution of different cell-free biosensors with high sensitivity.Cell-free synthetic biology as an emerging engineering strategy can be a better means applied to high-throughput screening of pharmaceutical proteins,detection of new pathogens,and other important health-care fields in the future.

关 键 词：无细胞合成生物学 医药蛋白质 便携式检测 无细胞生物传感器 

分 类 号：R319[医药卫生—基础医学]