岩藻糖氟化模拟类似物对乳腺癌细胞增殖、侵袭的影响及机制研究  

Effect and Mechanism of Fucose Fluoride Analogue Suppresses Breast Cancer Cell Proliferation and Migration

作　　者：周颖[1,2] 严伟 李晴宇 董蓉 马志媛 林能明 ZHOU Ying;YAN Wei;LI Qingyu;DONG Rong;MA Zhiyuan;LIN Nengming(Affiliated Hangzhou First People’s Hospital,Zhejiang University,School of Medicine,Department of Clinical Pharmacy,Hangzhou 310006,China;Affiliated Hangzhou First People’s Hospital,Zhejiang University,School of Medicine,Key Laboratory of Clinical Cancer Pharmacology and Toxicology Research of Zhejiang Province,Hangzhou 310006,China)

机构地区：[1]浙江大学医学院附属杭州市第一人民医院,药学部临床药学科,杭州310006 [2]浙江大学医学院附属杭州市第一人民医院,浙江省临床肿瘤药理与毒理学研究重点实验室,杭州310006

出　　处：《中国现代应用药学》2020年第2期143-148,共6页Chinese Journal of Modern Applied Pharmacy

基　　金：国家自然科学基金项目(31900922);浙江省医学重点学科(省市共建，浙卫办[2018]2号);杭州市医学重点学科(杭卫计发[2017]51号)

摘　　要：目的研究岩藻糖的氟化模拟类似物2-氟-L-岩藻糖(2-fluoro-L-fucose,2FF)对乳腺癌MDA-MB231细胞增殖和侵袭的影响,并探讨其可能的作用机制。方法凝集素染色法检测2FF处理后乳腺癌细胞中核心岩藻糖的表达情况;CCK-8法测定2FF处理对乳腺癌细胞增殖能力的影响;Transwell实验法测定2FF处理后乳腺癌侵袭能力的变化;Western blotting分析Hippo信号通路相关蛋白的表达情况。结果 2FF可显著抑制乳腺癌MDA-MB231细胞中核心岩藻糖的表达,呈剂量和时间依赖性;2FF处理后可以显著抑制乳腺癌MDA-MB231细胞的增殖和侵袭;Westernblotting结果显示,2FF使用后,pYAP、pLATS、pMST1/2表达下降,总体YAP、LATS、MST1表达未见明显改变。结论 2FF可以抑制核心岩藻糖在乳腺癌细胞中的表达,并抑制乳腺癌细胞的增殖和迁移;其机制可能与Hippo信号通路的调节有关。OBJECTIVE To investigate effect of fucose fluoride analogue(2-fluoro-L-fucose,2 FF) on the proliferation and invasion of breast cancer MDA-MB231 cells,and to explore the possible mechanism.METHODS Lectin blot was performed to detect the expression of core fucose in breast cancer cells after 2 FF treatment.CCK-8 was used to determine the effect of 2 FF treatment on the proliferation of breast cancer cells.The changes of invasive abilities of breast cancer cells after 2 FF treatment were determined by Transwell assay and the expression of Hippo signaling pathway-related proteins were checked by Western blotting.RESULTS 2 FF suppressed core fucosylation in the breast cancer,in a dose-and time-dependent manner.After treated by 2 FF,the cell proliferation and migration was significantly inhibited.The expression of pYAP,pLATS,pMST1/2 was significantly decreased in the 2 FF treated cells compared with the control cells.However,the expression of YAP,LATS,MST1 had little changes.CONCLUSION 2 FF inhibit the expression of core fucosylation in the breast cancer cells,and inhibit the cell proliferation and migration.The mechanism may be related to the regulation of Hippo signaling pathway.

关 键 词：岩藻糖氟化模拟类似物 乳腺癌 细胞增殖 细胞迁移 

分 类 号：R956.2[医药卫生—药学]