固定时间对中性缓冲福尔马林固定人体组织STR基因座检出率的影响  

Effect of Fixation Time on Detecting STR loci from Human Tissues Fixed by Neutral Buffered Formalin

作　　者：徐瑞光 王林[1] 张启荣 陈建军 伊小凯 卢晓军[1] 郭忠辉 孙敬[2] 王利华 张新生 XU Ruiguang;WANG Lin;ZHANG Qirong;CHEN Jianjun;YI Xiaokai;LU Xiaojun;GUO Zhonghui;SUN Jing;WANG Lihua;ZHANG Xinsheng(Baotou Public Security Bureau,Baotou 014030,Inner Mongolia,China;Institute of Forensic Science,Ministry of Public Security,Beijing 100038,China)

机构地区：[1]包头市公安局刑侦支队,内蒙古包头014030 [2]公安部物证鉴定中心,北京100038

出　　处：《刑事技术》2020年第1期30-34,共5页Forensic Science and Technology

基　　金：公安部技术研究计划项目（2016JSYJB03）

摘　　要：目的研究固定时间对福尔马林缓冲溶液固定的人体组织DNA的STR基因座检出率的影响。方法将心、肝、脾、肺、肾五种人体组织于室温在10%福尔马林中性缓冲溶液中固定,并在不同时间取样。磁珠法提取组织DNA,用AmpFSTR identifiler plus、AmpFSTR MiniFiler试剂盒分别进行扩增,扩增产物在ABI 3130xL遗传分析仪上进行检测,用GeneMapperIDv3.2对STR基因座检出情况作分析。结果随着固定时间延长,组织的STR基因座检出率下降明显。固定第30天时,使用Identifiler plus扩增系统可检出约80%的STR基因座,固定60、90d,STR基因座检出率分别下降到60%、20%左右。固定60d内的组织,使用Mini?ler扩增系统可检出全部9个miniSTR基因座。心肺两种组织表现出较高的STR基因座检出率。结论随着固定时间的延长,组织的STR基因座检出率下降明显,Minifiler可以作为常规扩增系统的有效补充,以提高长片段STR基因座的检出率。Objective To investigate the detection rate of STR loci from human tissues fixed for different time with buffered formalin solution. Methods Five human tissues, heart, liver, spleen, lung and kidney, were fixed in 10% neutral formalin buffer under room temperature and sampled at different time. DNA was extracted from the above formalin-fixed tissues by immuno-magnetic beads, then subjected to PCR amplification with both Amp FSTR?identifiler? plus kit and Amp FSTR?minifiler? plus kit. Through ABI 3130 x L genetic analyzer to detect the PCR products, the STR loci were analyzed by Gene Mapper IDv3.2. Results With the elongation of fixation time, the detection rate was reduced of STR loci from tissues. A majority of STR loci could be detected by the Identifiler plus amplification system within 30 days after fixation although the detection rate gradually decreased from about 60% to 20% when the fixation time extended to 60 ~ 90 days. All of the 9 STR loci could be detected by the minifiler amplification system within 60 days of fixation. Both heart and lung, amongall the 5 detected human tissues, had higher STR-loci detection rate. Conclusions The detection rate of STR loci decreased significantly with the prolongation of fixed time. Minifiler kit is capable of an effective supplement to the conventional amplification choice because of its improvement for the detection rate of larger fragmental STR loci.

关 键 词：法医物证学 缓冲福尔马林固定剂 STR分型 

分 类 号：DF795.2[医药卫生—法医学]