UPLC-MS/MS快速筛查尿样中61种毒品及滥用药物  

Rapid and Simultaneous Quantification of 61 Abused Drugs in Urine by UPLC-MS/MS

作　　者：刘帅 赵蒙[2] 杨发震 李绍鹏 王朝虹[2] 李虹 LIU Shuai;ZHAO Meng;YANG Fazhen;LI Shaopeng;WANG Zhaohong;LI Hong(Institute of Forensic Medicine,Kunming Medical University,Kunming 650500,China;Center of Procuratoral Technology and Information,Supreme People’s Procuratorate,Beijing 100144,China;Yuxi Public Security Bureau,Yuxi 653100,Yunnan,China;Key Laboratory of Narcotics Assay and Control Technology,Ministry of Public Security,Kunming 650228,China)

机构地区：[1]昆明医科大学法医学院,昆明650500 [2]最高人民检察院检察技术信息研究中心,北京100040 [3]玉溪市公安局,云南玉溪653100 [4]毒品分析及禁毒技术公安部重点实验室,昆明650228

出　　处：《刑事技术》2020年第1期45-50,共6页Forensic Science and Technology

基　　金：公安部科技强警基础工作专项项目（2016GABJC50）

摘　　要：目的建立了一种快速检测尿样中61种毒品及滥用药物的超高效液相色谱串联质谱(UPLC-MS/MS)方法。方法尿样按19(体积比)经甲醇沉淀蛋白、离心处理,采用电喷雾离子源正离子(ESI+)模式和多反应检测(MRM)模式进行质谱分析。结果61种化合物在0.1~10ng/mL浓度范围内均获得良好的线性,相关系数均在0.99以上,该方法的提取回收率分布在90.14%~129.72%之间,最小检测限可达0.05ng/mL。结论本方法灵敏、简便、快速,适用于尿样中毒品及滥用药物的定性定量分析。Objective A rapid and sensitive method of ultra-performance liquid chromatography coupled with mass spectrometry(UPLC-MS/MS) was to develop for simultaneous determination of 61 abused drugs in urine. Methods Urine sample was precipitated with methanol under the ratio of 1:9(V:V), afterwards centrifuged at 8500 r/min for 10 min, with the supernatant being analyzed through UPLC-MS/MS in coalescence of an electrospray ionization(ESI) source. The samples were measured in the mode of electrospray positive ionization(ESI+) and multiple reaction monitoring(MRM). Results The 61 drugs were linear in the range of 0.1~10 ng/m L, with their coefficients higher than 0.99. The extraction recoveries ranged from 90.14%~129.72% for all of the analytes, and their limits of detection(LODs) were 0.05 ng/m L. Conclusions The method presented here is validated to be simple, rapid, sensitive and accurate for determination of trace drugs and metabolites in urine samples.

关 键 词：法医毒物分析 滥用药物 超高效液相色谱-串联四级杆质谱 尿样 

分 类 号：DF795.1[医药卫生—法医学]