4种固相萃取柱筛选血中67种毒（药）物  

Performance about Four Sorts of Solid-phase Extraction Column to Select 67 Kinds of Poison(Drug) in Blood

作　　者：吕惊晗 蒋华宇 LV Jinghan;JIANG Huayu(Technology Division of Criminal Police Detachment of Qingdao Public Security Bureau,Qingdao 266001,Shandong,China)

机构地区：[1]青岛市公安局刑警支队技术处

出　　处：《刑事技术》2020年第1期64-66,共3页Forensic Science and Technology

摘　　要：目的考察常见的4种固相萃取柱对血中同时添加的67种毒(药)物的回收率,从而选择最佳固相萃取柱,优化前处理方法。方法根据4种固相萃取柱填料及保留机制的不同,实现对67种毒(药)物的分离、纯化和富集,基于不同的吸附效果,影响到每种药物不同的回收率,经GC/MS分析后得出具体数值,根据添加系列浓度的标准物质,考察优选柱子对每种药物的检出限。结果通过以空白血为基体的加标实验,绝大多数药物的方法检出限均在0.2~2μg/mL之间,回收率均在50%~150%之间。结论该方法简便灵敏、快速实用、净化效果佳,已在数起毒物化验案件的检验中发挥了很好的作用。Objective To test the recovery rate of 67 kinds of adding-into-blood poison(drug) by four sorts of solidphase extraction(SPE) column so that the best pretreatment method can be optimized from the chosen SPE column. Methods According to the mechanisms and fillers of the four sorts of SPE column, the selected 67 kinds of poison/drug were separated, purified and enriched. With the discrepant adsorptive effect on recovery of each drug, the performance of each sort of SPE column was assessed through GC/MS analysis to get the specific values(limit of detection of each drug) from the added standard substances of series of concentrations. Results From the blank blood as the spiked matrix, the detection limit of the majority of drugs was between 0.2~2 μg/m L, with the recoveries being among 50% and 150%. Conclusions The method is simple, rapid, sensitive, practical, showing good purification effect, having played good role in several forensic toxicological cases.

关 键 词：毒(药)物分析 固相萃取 气相色谱-质谱联用法(GC/MS) 回收率 前处理方法优化 

分 类 号：DF795.1[医药卫生—法医学]