33种鱼类微型反向重复转座元件鉴定  

IDENTIFICATION OF MITE TRANSPOSONS IN 33 FISH GENOMES

作　　者：胡竞文 邵峰 赵连鹏 韩民锦[2] 彭作刚 HU Jing-Wen;SHAO Feng;ZHAO Lian-Peng;HAN Min-Jin;PENG Zuo-Gang(Key Laboratory of Freshwater Fish Reproduction and Development(Ministry of Education),School of Life Sciences,Southwest University,Chongqing 400715,China;State Key Laboratory of Silkworm Genome Biology,Southwest University,Chongqing 400715,China)

机构地区：[1]西南大学生命科学学院,淡水鱼类资源与生殖发育教育部重点实验室,重庆400715 [2]西南大学家蚕基因组生物学国家重点实验室,重庆400715

出　　处：《水生生物学报》2020年第1期1-9,共9页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金(31572254和31872204)资助~~

摘　　要：微型反向重复转座元件(MITEs)是一类短的非自主DNA转座子,其分布的位置会对宿主产生影响。文章使用生物信息学的方法对无颌类、软骨鱼纲、肉鳍鱼纲和辐鳍鱼纲鱼类基因组进行了MITEs预测,最终在33种鱼类基因组中鉴定出2433个M I T E s家族。文章发现鱼类基因组中M I T E s含量存在较大差异(0.11%—21.18%),并且MITEs含量与鱼类基因组大小呈正相关关系。根据末端重复序列(TIRs)和靶位点重复序列(TSDs)的特征将MITEs分为10个超家族,其中TC1-Mariner超家族的含量最高。MITEs在鱼类基因组中的插入事件主要发生在4百万年前至今,大多数物种的MITEs在2百万—0.5百万年前发生了爆发式扩增。鱼类基因组中的MITEs多数插入到基因内部或附近,这些转座子可能在基因的表达调控方面存在重要作用。Miniature inverted-repeat transposable elements(MITEs),a group of short and non-autonomous DNA transposons,are widely present in eukaryotic genomes.The location of the MITEs distribution would affect the host.In this study,MITEs in 33 fish genomes of the agnatha,chondrichthyes,sarcopterygii and actinopterygii were predicted and analyzed using bioinformatics approach.Ultimately,2433 MITEs were identified in 33 fish genomes.MITEs content in 33 fish genomes varied from 0.11%to 21.18%,and MITEs content was positively correlated with fish genome size.MITEs were classified into 10 super families according to different terminal inverted repeats(TIRs)and target site duplications(TSDs)with the largest family of TC1-Mariner superfamily.The insertion of MITEs into the fish genome was mainly happened 4 million years ago to now,and most species had an explosive expansion between 2—0.5 million years ago.A number of fish MITEs were inserted into or near the genes,which may play an important role in the regulation of gene expression.

关 键 词：微型反向重复转座元件(MITEs) 鱼类 插入时间 分布位置 

分 类 号：Q344.1[生物学—遗传学]