Transcriptome profiling reveals phase-specific gene expression in the developing barley inflorescence  

作　　者：Huiran Liu Gang Li Xiujuan Yang Hendrik N.J.Kuijer Wanqi Liang Dabing Zhang 

机构地区：[1]Joint International Research Laboratory of Metabolic&Developmental Sciences,Shanghai Jiao Tong University–University of Adelaide Joint Centre for Agriculture and Health,State Key Laboratory of Hybrid Rice,School of Life Sciences and Biotechnology,Shanghai Jiao Tong University,Shanghai 200240,China [2]School of Agriculture,Food and Wine,The University of Adelaide,South Australia 5064,Australia

出　　处：《The Crop Journal》2020年第1期71-86,共16页作物学报（英文版）

基　　金：supported by Australian Research Council(DP170103352);an Australia-China Science and Research Fund Joint Research Centre grant ACSRF48187;Start-up funding(Australia,13114779,62117250)for Dabing Zhang from the School of Agriculture,Food and Wine,The University of Adelaide;the Innovative Research Team,Ministry of Education of China;the 111 Project(B14016)

摘　　要：The shape of an inflorescence varies among cereals,ranging from a highly branched panicle in rice to a much more compact spike in barley(Hordeum vulgare L.)and wheat(Triticum aestivum L.).However,little is known about the molecular basis of cereal inflorescence architecture.We profiled transcriptomes at three developmental stages of the barley main shoot apex—spikelet initiation,floral organ differentiation,and floral organ growth—and compared them with those from vegetative seedling tissue.Transcript analyses identified 3688 genes differentially transcribed between the three meristem stages,with a further 1394 genes preferentially expressed in reproductive compared with vegetative tissue.Coexpression assembly and Gene Ontology analysis classified these 4888 genes into 28 clusters,revealing distinct patterns for genes such as transcription factors,histone modification,and cell-cycle progression specific for each stage of inflorescence development.We also compared expression patterns of VRS(SIX-ROWED SPIKE)genes and auxin-,gibberellic acid-and cytokinin-associated genes between two-rowed and six-rowed barley to describe regulators of lateral spikelet fertility.Our findings reveal barley inflorescence phase-specific gene expression,identify new candidate genes that regulate barley meristem activities and flower development,and provide a new genetic resource for further dissection of the molecular mechanisms of spike development.The shape of an inflorescence varies among cereals,ranging from a highly branched panicle in rice to a much more compact spike in barley(Hordeum vulgare L.) and wheat(Triticum aestivum L.).However,little is known about the molecular basis of cereal inflorescence architecture.We profiled transcriptomes at three developmental stages of the barley main shoot apex — spikelet initiation,floral organ differentiation,and floral organ growth — and compared them with those from vegetative seedling tissue.Transcript analyses identified3688 genes differentially transcribed between the three meristem stages,with a further1394 genes preferentially expressed in reproductive compared with vegetative tissue.Coexpression assembly and Gene Ontology analysis classified these 4888 genes into 28 clusters,revealing distinct patterns for genes such as transcription factors,histone modification,and cell-cycle progression specific for each stage of inflorescence development.We also compared expression patterns of VRS(SIX-ROWED SPIKE) genes and auxin-,gibberellic acid-and cytokinin-associated genes between two-rowed and six-rowed barley to describe regulators of lateral spikelet fertility.Our findings reveal barley inflorescence phase-specific gene expression,identify new candidate genes that regulate barley meristem activities and flower development,and provide a new genetic resource for further dissection of the molecular mechanisms of spike development.

关 键 词：Inflorescence meristem TRANSCRIPTOME Gene expression HORMONES BARLEY 

分 类 号：S512.3[农业科学—作物学]