不同来源绿僵菌对油桐鹰尺蠖幼虫的致病力比较  

Comparison of Pathogenicity of Different Metarhizium anisopliae Strains against Biston suppressaria Larvae

作　　者：蔡守平[1] 何学友[1] 林和再 宋海天 郑宏 尹丽钦 何金源 CAI Shouping;HE Xueyou;LIN Hezai;SONG Haitian;ZHENG Hong;YIN Liqin;HE Jinyuan(Fujian Academy of Forestry/The Key Laboratory of Timber Forest Breeding and Cultivation for Mountainous Areas in Southern China,Fuzhou,Fujian 350012,China;Forestry Bureau of Zhangpu County,Zhangpu,Fujian 363200,China;Forestry Diseases and Pests Control Station of Xinluo District,Longyan City,Longyan,Fujian 364000,China;Forestry Bureau of Minqing County,Minqing,Fujian 335080,China;Tianma National Forest Farm,Pinghe,Fujian 363704,China)

机构地区：[1]福建省林业科学研究院/国家林业局南方山地用材林培育重点实验室,福建福州350012 [2]漳浦县林业局,福建漳浦363200 [3]龙岩市新罗区森林病虫害防治检疫站,福建新罗364000 [4]闽清县林业局,福建闽清335080 [5]福建省平和天马国有林场,福建平和363704

出　　处：《林业与环境科学》2019年第6期44-49,共6页Forestry and Environmental Science

基　　金：福建省属公益类科研院所基本科研专项(2017R1011-8);国家重点研发计划子课题(2018YFD0600202);福建省林业科学研究项目(闽林科〔2017〕3号);国家林业局南方山地用材林培育重点实验室资助

摘　　要：油桐鹰尺蟻Biston suppressaria(Guenee)是一种杂食性食叶害虫,近年来在速生枚■树林分中大面积发生,造成较大的损失。文中研究了不同来源金龟子绿僵菌Metarhizium anisopliae菌株生长与产孑包特性及对油桐鹰尺蟆幼虫的致病力,旨在筛选出高效防治该虫的生物资源。试验结果表明:不同来源的绿僵菌菌株间菌落生长速度和产葩量差异显著,MaZPTR-01菌株15d的菌落直径为6.5 cm,显著较其它菌株大;MaFZ-13和MaZPTR-01菌株培养15 d产胞量最高,分别为1.33 x 108子cm和1.28x10蔦包子-cm'2o致病力测定结果表明:不同菌株对油桐鹰尺蟆幼虫的致病力存在显著差异,其中接种MaFZ-13和MaZPTR-01菌株的幼虫校正死亡率、僵虫率均较其它菌株高,接种后15 d幼虫校正死亡率分别达到94.5%、92.7%,僵虫率分别为75%、61.7%;这2个菌株的致死中时(LT50)显著比其它菌株短,分别为4.34和4.52d。绿僵菌MaFZ-13和MaZPTR-01菌株对油桐鹰尺護幼虫的致病力强,且具有良好的生产性状,可作为该虫的生物防治资源进一步开发应用。Biston suppressaria is a omnivorous defoliator which occurred in large area of eucalypt forest and caused enormous losses.For search the biocontrol resource for this pest,pathogenicity of different Metarhizium anisopliae strains against B.suppressaria larvae was compared.The results showed that there were significant differences in hyphal growth and sporulation among different strains.Colony diameter of MaZPTR-01 strain was 6.5 cm 15 days after cultured on plate medium,which was significant bigger than other strains.Sporulation of MaFZ-13 and MaZPTR-01 were 1.33 x 108 and 1.28 x 108spores•cm'2 when 15 days after cultured on plate medium.Results of bioassay showed that virulences of different M.anisopliae strains against B.suppressaria larvae were significantly different.The corrected mortality and muscardine cadaver rates inoculated with MaFZ-13 and MaZPTR-01 were significant higher than other strains.The median lethal time(LT50)of these two strains were shorter than other strains,which were 4.34 and 4.52 d respectively.The results showed that MaFZ-13 and MaZPTR-01 were high virulent strains against B.suppressaria larvae with good production trait,which could be further researched and developed as a potential biocontrol agent against B.suppressaria.

关 键 词：金龟子绿僵菌 油桐鹰尺蟻 致死中时(LT50) 僵虫率 枝树 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治] S763.38[农业科学—植物保护]