水稻白叶枯病菌wxoC和wxoD基因功能的初步研究  

Preliminary Study on Function of wxoC and wxoD Genes in Xanthomonas oryzae pv. oryzae

作　　者：倪哲[1] 韩倩[1] 陈龙 韦新利 李建媛 黄胜[1] Ni Zhe;Han Qian;Chen Long;Wei Xinli;Li Jianyuan;Huang Sheng(College of Life Science and Technology,Guangxi University,Nanning,530004)

机构地区：[1]广西大学生命科学与技术学院

出　　处：《基因组学与应用生物学》2019年第11期5023-5030,共8页Genomics and Applied Biology

基　　金：广西自然科学基金项目(2015GXNSFCB139002)资助

摘　　要：水稻白叶枯病菌(Xanthomonas oryzae pv.oryzae,Xoo)是目前发现的危害较大的水稻病原细菌之一,可导致水稻减产。本研究以广西菌株Xoo K74为出发菌株,通过在wxoC和wxoD双基因功能被破坏的突变体中分别回补wxoC或wxoD基因发现:wxoC基因影响病菌的致病力,降低了菌体对有机溶剂的耐受性,部分影响细菌生物膜的产生和胞外多糖的分泌;wxoD基因影响细菌胞外多糖的合成、运动能力、对渗透压的耐受性和细菌的生长情况,并且部分影响生物膜的产生,但该基因对致病力贡献很小,具体原因有待进一步的研究。Xanthomonas oryzae pv.oryzae(Xoo)is one of the most harmful bacterial pathogens found in rice and can cause rice production reduction.In this study,Guangxi strain Xoo K74 was used as the starting strain.wxoC or wxoD can be respectively fed back through the mutant which is damaged by the functions of the wxC and the wxoD doublegene.It was found that the wxoC gene influences the virulence of pathogens and reduce the bacterias’tolerance on organic solvents.Moreover it partially affects bacterial biofilm production and EPS secretion.The wxoD gene affects bacterial LPS synthesis,motility,tolerance to osmotic pressure,bacterial growth and partially affects biofilm production.But the gene contributes very little to the virulence.The specific reason remains need to be further studied.

关 键 词：水稻白叶枯病菌 致病力 脂多糖 wxoC wxoD 

分 类 号：S435.111.47[农业科学—农业昆虫与害虫防治]