白介素10对人成熟树突状细胞蛋白表达的影响  

Effects of Interleukin-10 on the Protein Expression of Human Mature Dendritic Cells

作　　者：黄江涛[1,3] 赵雪 刘江丽[1,2] 王赟 刘丽娜 胡祖权[1,2] 曾柱 Huang Jiangtao;Zhao Xue;Liu Jiangli;Wang Yun;Liu Lina;Hu Zuquan;Zeng Zhu(Immune Cells and Antibody Engineering Research Center of Guizhou Province,Key Laboratory of Biology and Medical Engineering,Guizhou Medical University,Guiyang,550025;School of Biology and Engineering,Guizhou Medical University,Guiyang,550025;School of Basic Medical Sciences,Guizhou Medical University,Guiyang,550025)

机构地区：[1]贵州省免疫细胞与抗体工程研究心,贵州医科大学生物与医学工程重点实验室,贵阳550025 [2]贵州医科大学生物与工程学院,贵阳550025 [3]贵州医科大学基础医学院,贵阳550025

出　　处：《基因组学与应用生物学》2019年第11期5250-5257,共8页Genomics and Applied Biology

基　　金：国家自然科学基金(31771014,31660258,11762006);贵州省高层次创新型人才培养计划(黔科合平台人才(2016)5676);贵州省科技计划重点项目(黔科合基础(2018)1412)共同资助

摘　　要：利用人外周血淋巴细胞分离液密度梯度离心分离CD14^+的单核细胞,并通过IL-4、GM-CSF、LPS、IFN-γ等重组细胞因子在体外将其诱导为成熟树突状细胞(mature dendritic cells,mDCs),利用10 ng/mL白介素10(interleukin-10,IL-10)处理mDCs 4 h,双向电泳联合MALDI-TOF/TOF MS技术分析差异表达蛋白。结果显示共筛选出35个差异蛋白点,其中21个上调,14个下调,它们的功能主要涉及糖代谢、HIF信号转导通路、细胞骨架和免疫功能等。因此,IL-10可能通过调控mDCs的代谢过程以及细胞骨架或运动能力等相关蛋白来影响其生物学功能,为进一步深入研究IL-10对mDCs的影响具有潜在作用。CD14^+monocytes were isolated by liquid density gradient centrifugation and induced into mature dendritic cells(mDC s)in vitro by recombinant cytokines of IL-4,GM-CSF,LPS and IFN-γ.The mDCs were treated with 10 ng/mL interleukin-10(IL-10)for 4 h.Then,two-dimensional electrophoresis and MALDI-TOF/TOF MS were deployed to analyze the differentially expressed proteins.The results showed that a total of 35 differential protein spots were screened out,of which 21 were up-regulated and 14 were down-regulated.Their functions were mainly related to glucose metabolism,HIF signal transduction pathway,cytoskeleton and immune function.Thus,IL-10 may affect the biological function of mDCs by regulation the metabolism process and related proteins such as cytoskeleton and migratory capability.These results play a potential role for further exploring the effects of IL-10 on mDCs.

关 键 词：白介素10 树突状细胞 双向电泳 差异表达蛋白 

分 类 号：R392[医药卫生—免疫学]