地龙降压胶囊对自发性高血压大鼠凝血相关的血小板miRNAs的影响  

Effect of Dilong antihypertension Capsule on miRNAs of Coagulation-related Platelets in Spontaneously Hypertensive Rats

作　　者：王碧莹 李应东[1] 刘永琦[1,2] 张小平[1,2] 岳晓华 李长天[1] 谢晓蓉[1] 孙少伯[1] WANG Biying;Li Yingdong;LIU Yongqi;ZHANG Xiaoping;YUE Xiaohua;LI Changtian;XIE Xiaorong;SUN Shaobo(Gansu University of Chinese medicine,Lanzhou 730000,China;Dunhuang Medicine and Transformation Key Laboratory of Ministry of Education,Gansu University of Chinese medicine,Lanzhou 730000,China)

机构地区：[1]甘肃中医药大学,甘肃兰州730000 [2]甘肃中医药大学敦煌医学与转化省部共建教育部重点实验室,甘肃兰州730000

出　　处：《中国民族民间医药》2020年第1期13-20,34,共9页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：甘肃省中医药管理局自然科学基金资助课题(GZK-2016-11);国家自然科学基金资助课题(81360345)

摘　　要：目的:通过地龙降压胶囊对SHR大鼠凝血相关的血小板miRNAs表达的影响,探讨地龙降压胶囊的降压机制。方法:SHR大鼠分为5组:正常对照组、肝素阳性对照组、地龙降压胶囊低、中、高剂量组。药物高、中、低组分别灌胃地龙降压胶囊100 mg/kg、50 mg/kg、25 mg/kg。15 d后取血,实时荧光定量PCR(RT-PCR)方法检测5种miRNAs的表达水平。结果:与正常对照组相比,地龙降压胶囊干预组与血小板活化相关的miRNA-223、miRNA-96和聚集相关的miRNA-130a的表达量明显改变,而与血小板生成有关的miRNA-155、miRNA34a-5p的表达量无明显变化。结论:地龙降压胶囊可能主要通过影响血小板活化与聚集相关的miRNAs发挥抗凝血降压作用。Objective To explore the hypotensive mechanism of Dilong antihypertension Capsule, based on the investigations of the expressions of miRNAs coagulation-related platelets after the administration of Dilong antihypertension Capsule. Methods SHR rats were randomly divided into 5 groups: normal control group, heparin-positive control group, low-dose group of Dilong antihypertension Capsule, medium-dose group of Dilong antihypertension Capsule, high-dose group of Dilong antihypertension Capsule. The high, medium and low-dose groups were respectively given Dilong antihypertension Capsule 100 mg/kg, 50 mg/kg and 25 mg/kg. Bloods were collected 15 days after administration, and the expressions of five miRNAs were detected by real-time fluorescence quantitative PCR(RT-PCR). Results Compared with the control group, the expressions of platelet activation-related miRNA-223, miRNA-96 and conglomeration-related miRNA-130 a in Dilong antihypertension Capsule-treated groups changed significantly, while there was no obvious change in the expressions of miRNA-155 and miRNA34 a-5 p related to thrombocytopoiesis. Conclusion Dilong antihypertension Capsule may play an anticoagulant and hypotensive role mainly by affecting the miRNAs expressions of platelet activation and aggregation.

关 键 词：地龙降压胶囊 MIRNAS 凝血相关 

分 类 号：R285.5[医药卫生—中药学]