桑皮素对人宫颈癌Hela细胞增殖与凋亡的影响  

Effects of morusin on the proliferation and apoptosis of human cervical cancer Hela cells

作　　者：左亚奇 高志红 庞策 刘赛娜 张晓丽[1] 孙转友 甄攀[1] ZUO Ya⁃qi;GAO Zhi⁃hong;PANG Ce;LIU Sai⁃na;ZHANG Xiao⁃li;SUN Zhuan⁃you;ZHEN Pan(Hebei North University,Zhangjiakou 075000,China)

机构地区：[1]河北北方学院

出　　处：《中成药》2020年第2期317-322,共6页Chinese Traditional Patent Medicine

基　　金：河北省高等学校科学技术研究项目(ZD2016016);2018年度河北北方学院一般项目(YB2018030)

摘　　要：目的探讨桑皮素对人宫颈癌Hela细胞的增殖抑制及其凋亡诱导作用。方法用10、15、20μmol/L桑皮素作用Hela细胞24 h后,在扫描电镜、透射电镜及倒置显微镜下观察细胞形态学变化;利用MTT法检测桑皮素对Hela细胞的增殖的影响;应用Hoechst单染实验检测Hela细胞的凋亡情况;采用qRT⁃PCR和Western blot检测Bax、Bcl⁃2 mRNA及蛋白的表达。结果桑皮素(2.5、5、7.5、10、15、20、25μmol/L)处理Hela细胞24、48 h,能明显的抑制Hela细胞增殖(P<0.05),且呈浓度依赖性。桑皮素(10、15、20μmol/L)处理Hela细胞24 h,细胞呈现早期凋亡。与0μmol/L桑皮素组比较,桑皮素(10、15、20μmol/L)能显著上调Bax mRNA及蛋白表达(P<0.05),下调Bcl⁃2 mRNA及蛋白表达(P<0.05)。结论桑皮素通过上调Bax表达和下调Bcl⁃2表达,从而诱导Hela细胞发生凋亡。AIM To investigate the effects of morusin on proliferation inhibition and apoptosis induction of hu⁃man cervical carcinoma Hela cells.METHODS Hela cells treated with 10,15 and 20μmol/L of morusin for 24 h had their morphology observed under inverted microscope,transmission electron microscope and scanning electron microscope.The effect of morusin on Hela cells proliferation was detected by MTT assay,and its impact on the apoptosis was performed by Hoechst single staining assay.The expressions of Bax and Bcl⁃2 mRNA and protein were detected by RT⁃qPCR and Western blot.RESULTS 24 h and 48 h treatment of Hela cells with morusin(2.5,5,7.5,10,15,20,25μmol/L)brought forth a significant concentration⁃dependent inhibition of Hela cells proliferation(P<0.05).Hela cells exposed to 24 h morusin(10,15,20μmol/L)showed early apoptosis.Compared with 0μmol/L morusin,morusin(10,15,20μmol/L)contributed significant up⁃regulation of Bax mRNA and protein expression(P<0.05),and down⁃regulation of Bcl⁃2 mRNA and protein expression(P<0.05).CONCLUSION Morusin can induce apoptosis of Hela cells by up⁃regulating Bax expression and down⁃regulating Bcl⁃2 expression.

关 键 词：桑皮素 HELA细胞 增殖 凋亡 

分 类 号：R285􀆰[医药卫生—中药学]