高效液相色谱波长切换法同时测定清凉膏药中7种成分的含量  

Simultaneous determination of 7 compositions in Qingliang plaster by HPLC wavelength switching method

作　　者：吴丽莎 朱新华 黄春兰 赵英 李雄杰 WU Li-sha;ZHU Xin-hua;HUANG Chun-lan;ZHAO Ying;LI Xiong-jie(Department of Pharmacy,Pingshan District People's Hospital of Shenzhen,Shenzhen Guangdong 518118;Department of Pharmacy,Pingshan State Medical Hall Hospital,Beijing University of Chinese Medicine,Shenzhen Guangdong 518118;Chemistry and Chemical Engineering College,Wuhan Textile University,Wuhan 430200)

机构地区：[1]深圳I市坪山区人艮医院药剂科,广东深圳518118 [2]北京中医药大学坪山国医堂药剂科,广东深圳518118 [3]武汉纺织大学化学与化工学院,武汉430200

出　　处：《中南药学》2019年第12期2135-2139,共5页Central South Pharmacy

基　　金：坪山区卫生系统科研项目（No.201705）

摘　　要：目的建立高效液相色谱波长切换法测定清凉膏药中氧化前胡素、欧前胡素、异欧前胡素、梓醇、毛蕊花糖苷、焦地黄苯乙醇苷B1、马替诺皂苷的含量。方法采用Dikma Diamonsil C18色谱柱(250mm×4.6 mm,5μm),柱温25℃;采用甲醇-乙腈(3∶1)与0.1%磷酸溶液为流动相,梯度洗脱,流速0.9 mL·min^-1;氧化前胡素、欧前胡素、异欧前胡素的检测波长为310 nm,梓醇的检测波长为210 nm,毛蕊花糖苷、焦地黄苯乙醇苷B1、马替诺皂苷的检测波长为330 nm。结果氧化前胡素、欧前胡素、异欧前胡素、梓醇、毛蕊花糖苷、焦地黄苯乙醇苷B1、马替诺皂苷的线性范围分别为2.17~43.40 mg·L^-1(r=0.9991),1.76~35.20 mg·L^-1(r=0.9998),0.88~17.60 mg·L^-1(r=0.9993),3.26~65.20mg·L^-1(r=0.9996),2.37~47.40 mg·L^-1(r=0.9999),1.19~23.80 mg·L^-1(r=0.9995),1.46~29.20 mg·L^-1(r=0.9994);平均加样回收率分别为98.3%(RSD=1.4%)、97.6%(RSD=1.1%)、97.0%(RSD=1.5%)、100.0%(RSD=0.69%)、99.2%(RSD=0.99%)、97.4%(RSD=1.0%)、98.4%(RSD=0.89%)。结论该方法操作便捷、重复性好,可用于清凉膏药的的定量分析和质量评价。Objective To simultaneously determine oxypeucedanin,imperatorin,isoimperatorin,catalpol,verbascoside,jionoside B1 and martinoside in Qingliang plaster by HPLC wavelength switching method.Methods Dikma Diamonsil C18(250 mm×4.6 mm,5μm)column was used and the column temperature was 25℃.Gradient elution was conducted with methanol-acetonitrile(3∶1)and 0.1%phosphoric acid solution,at the flow rate of 0.9 mL·min^-1.The detection wavelength for oxypeucedanin,imperatorin and isoimperatorin was 310 nm,that for catalpol was 210 nm,and that for verbascoside,jionoside B1 and martinoside was 330 nm.Results The linearity of oxypeucedanin,imperatorin,isoimperatorin,catalpol,verbascoside,jionoside B1 and martinoside were at 2.17-43.40 mg·L^-1(r=0.9991),1.76-35.20 mg·L^-1(r=0.9998),0.88-17.60 mg·L^-1(r=0.9993),3.26-65.20 mg·L^-1(r=0.9996),2.37-47.40 mg·L^-1(r=0.9999),1.19-23.80 mg·L^-1(r=0.9995),and 1.46-29.20 mg·L^-1(r=0.9994),respectively.The average recovery was 98.3%(RSD=1.4%),97.6%(RSD=1.1%),97.0%(RSD=1.5%),100.0%(RSD=0.69%),99.2%(RSD=0.99%),97.4%(RSD=1.0%),and 98.4%(RSD=0.89%).Conclusion The method is simple and repeatable,which can be used for the quantitative analysis and quality evaluation of Qingliang plaster.

关 键 词：清凉膏药 波长切换法 高效液相色谱 氧化前胡素 欧前胡素 异欧前胡素 梓醇 毛蕊花糖苷 焦地黄苯乙醇苷B1 马替诺皂苷 

分 类 号：R917[医药卫生—药物分析学]