MTHFR基因多态性与不明原因复发性流产的关系  

Relationship between unexplained recurrent spontaneous abortion and MTHFR gene polymorphism

作　　者：许雅娟[1] 班彦杰 冉利敏 余艳茹 翟闪闪[1] 孙宗宗 张淼 张婧喆 洪腾[1] 任利单 胡璐璐[1] 王彪 侯晓峰 石瑛[2] XU Yajuan;BAN Yanjie;RAN Limin;YU Yanru;ZHAI Shanshan;SUN Zongzong;ZHANG Miao;ZHANG Jingzhe;HONG Teng;REN Lidan;HU Lulu;WANG Biao;HOU Xiaofeng;SHI Ying(Department of Gynecology and Obstetrics,the Third Affiliated Hospital,Zhengzhou University,Zhengzhou 450052;Department of Clinical Laboratory,the Third Affiliated Hospital,Zhengzhou University,Zhengzhou 450052)

机构地区：[1]郑州大学第三附属医院妇产科,郑州450052 [2]郑州大学第三附属医院检验科,郑州450052

出　　处：《郑州大学学报（医学版）》2020年第1期112-115,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省医学科技攻关计划项目(201403110)

摘　　要：目的:探讨育龄期女性不明原因的复发性流产(URSA)与亚甲基四氢叶酸还原酶(MTHFR)基因多态性的关系。方法:采集230例有URSA史(URSA组)和264例正常育龄期女性(对照组)的口腔黏膜上皮细胞,利用荧光定量PCR技术检测MTHFR基因的C677T、A1298C位点的序列,比较2组2个基因位点的差异。结果:URSA组MTHFR C677T位点T等位基因频率高于对照组(P=0.046);2组MTHFR 1298位点等位基因频率和基因型频率比较,差异均有统计学意义(P<0.05);URSA组MTHFR 677CC/1298AC、677CT/1298AC、677TT/1298AA复合基因型频率均高于对照组。结论:MTHFR A1298C和C677T多态性与URSA的发生有关。Aim:To explore the relationship between unexplained recurrent spontaneous abortion(URSA)and methylene tetrahydrofolate reductase(MTHFR)gene polymorphism among women of child-bearing age.Methods:Buccal smears were collected from 230 URSA patients and 264 healthy controls.Sequences of MTHFR C677T and A1298C were determined by the technology of fluorescence quantitative PCR.Results:The frequency of T allele of MTHFR C677T in URSA group was significantly higher than that in control group(P=0.046).The frequency of allele and genotype of MTHFR 1298 showed significant difference between URSA group and control group(P<0.05).Compared with control group,the frequency of MTHFR 677CC/1298AC,677CT/1298AC,677TT/1298AA compound heterozygous genotype in URSA group was significantly higher.Conclusion:MTHFR A1298C and C677T gene polymorphism are related to URSA.

关 键 词：流产 亚甲基四氢叶酸还原酶 单核苷酸多态性 

分 类 号：R714.21[医药卫生—妇产科学]