SIRT1对大鼠血管平滑肌细胞衰老的影响  

Effects of SIRT1 on aging of vascular smooth muscle cells

作　　者：李书国[1,2] 童格[1,2] 叶明 邓娟娟[1,2] 邵文[1,2] LI Shuguo;TONG Ge;YE Ming;DENG Juanjuan;SHAO Wen(Institute of Geriatrics,Three Gorges University,Yichang,Hubei 443003;)Department of Geriatrics,Yichang Central People′s Hospital,Yichang,Hubei 443003)

机构地区：[1]三峡大学老年医学研究所,湖北宜昌443003 [2]宜昌市中心人民医院老年病科,湖北宜昌443003

出　　处：《郑州大学学报（医学版）》2020年第1期143-147,共5页Journal of Zhengzhou University(Medical Sciences)

基　　金：湖北省自然科学基金项目(2016CFC023)

摘　　要：目的:研究沉默信息调节因子1(SIRT1)对血管平滑肌细胞衰老的影响。方法:分离培养大鼠血管平滑肌细胞,取对数生长期细胞分为对照组、血管紧张素Ⅱ(AngⅡ)组、AngⅡ+Lv-NC组、AngⅡ+Lv-SIRT1组,用100 nmoL/L的AngⅡ诱导血管平滑肌细胞衰老,AngⅡ+Lv-NC和AngⅡ+Lv-SIRT1组血管平滑肌细胞分别转染阴性对照慢病毒载体和SIRT1过表达慢病毒载体,对照组细胞仅常规培养。采用qRT-PCR和Western blot检测细胞中SIRT1的表达。β-半乳糖苷酶染色检测细胞衰老情况,Western blot检测细胞中衰老相关蛋白P21、P53表达,PI单染法检测细胞周期变化。结果:衰老诱导后的血管平滑肌细胞β-半乳糖苷酶阳性率升高,细胞中P21、P53蛋白表达水平升高,G0/G1期细胞比例增多(P<0.05)。过表达SIRT1能够降低衰老诱导条件下血管平滑肌细胞β-半乳糖苷酶的阳性率,减少细胞中P21、P53蛋白表达,降低G0/G1期细胞比例(P<0.05)。结论:SIRT1能够抑制AngⅡ诱导的血管平滑肌细胞衰老。Aim:To study the effects of SIRT1 on the aging of vascular smooth muscle cell.Methods:Rat vascular smooth muscle cells were isolated and cultured.The logarithmic growth phase cells were divided into control group,AngⅡgroup,AngⅡ+Lv-NC group and AngⅡ+Lv-SIRT1 group.Aging of vascular smooth muscle cells was induced by 100 nmol/L AngⅡ.The cells of AngⅡ+Lv-SIRT1 and AngⅡ+Lv-NC groups were transfected with SIRT1 over expressed lentivirus vector and negative control lentivirus vector respectively.The cells in control group were only cultured.SIRT1 expression was detected by qRT-PCR and Western blot.β-galactosidase staining was used to detect cell aging,Western blot was used to detect the expressions of P21 and P53 protein,and PI single staining was used to detect cell cycle.Results:After AngⅡinduction,theβ-galactosidase positive rate was significantly increased in the cells,the expressions of P21 and P53 protein were significantly increased,the proportion of cells in G0/G1 phase was significantly increased(P<0.05).Overexpression of SIRT1 significantly reduced the positive rate ofβ-galactosidase,the expressions of P21 and P53 protein,and the proportion of cells in G0/G1 phase(P<0.05).Conclusion:SIRT1 can inhibit the aging of vascular smooth muscle cells induced by AngⅡ.

关 键 词：血管平滑肌细胞 SIRT1 衰老 细胞周期 大鼠 

分 类 号：R543.3[医药卫生—心血管疾病]