不同激素配比对紫花苜蓿幼苗4种外植体愈伤组织诱导效果的影响  

Effects of different hormone ratio on induction callus of four explants of Medicago sativa L. seedlings

作　　者：王立爽 孟令媛 王沛銮 周婷 唐碧瑶 邬婷婷 李波[1,2] WANG Lishuang;MENG Lingyuan;WANG Peiluan;ZHOU Ting;TANG Biyao;WU Tingting;LI Bo(School of Life Science and Agriculture Forestry,Qiqihar University,Qiqihar 161006,China;Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas,Qiqihar University,Qiqihar 161006,China)

机构地区：[1]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006 [2]齐齐哈尔大学抗性基因工程与寒地生物多样性保护黑龙江省重点实验室,黑龙江齐齐哈尔161006

出　　处：《高师理科学刊》2020年第1期51-53,76,共4页Journal of Science of Teachers＇College and University

基　　金：黑龙江省省属高等学校基本科研业务费科研项目（135209267,YSTSXK201886);2019年黑龙江省大学生创新创业训练计划项目（201910232095）;齐齐哈尔市科技计划项目(NYGG-201916)

摘　　要：为了探究不同激素对苜蓿无菌苗不同外植体诱导效果的影响,采用不同质量浓度激素配比对4种外植体进行愈伤组织诱导,筛选不同外植体最佳愈伤组织诱导培养基.结果表明,下胚轴最佳诱导培养基为MS+2,4-D 1.0 mg/L+6-BA 1.0 mg/L;茎最佳诱导培养基为MS+2,4-D 2.0 mg/L+6-BA 0.5 mg/L;子叶最佳诱导培养基为MS+2,4-D 1.0 mg/L+6-BA 0.5 mg/L;叶最佳诱导培养基为MS+2,4-D 1.0 mg/L+6-BA 0.75 mg/L.In order to explore the effect of different hormones on the induction of different explants of Medicago sativa L.seedlings,four kinds of explants were used to induce callus,and the best induction medium of different explants was selected.The result showed that,the best medium for hypocotyl induction was MS+2,4-D 1.0 mg/L+6-BA 1.0 mg/L.The best medium for stem induction was MS+2,4-D 2.0 mg/L+6-BA 0.5 mg/L.The best medium for cotyledon induction was MS+2,4-D 1.0 mg/L+6-BA 0.5 mg/L.The best medium for leaf induction was MS+2,4-D 1.0 mg/L+6-BA 0.75 mg/L.

关 键 词：紫花苜蓿 外植体 激素 愈伤组织 

分 类 号：Q94[生物学—植物学]