长尾仓鼠VKORC1基因的提取与扩增  

Extraction and Amplification of VKORC1 Gene in Cricetulus longicaudatus

作　　者：杨新根[1,2] 杨静[1] 张建珍[2] 郭红芳 王庭林[1] 邹波[1] 常文英[1] 侯玉[1] YANG Xin'gen;YANG Jing;ZHANG Jianzhen;GUO Hongfang;WANG Tinglin;ZOU Bo;CHANG Wenying;HOU Yu(Shanxi Key Laboratory of Integrated Pest Management in Agriculture,Institute of Plant Protection,Shanxi Academy of Agricultural Sciences,Taiyuan 030031,China;Institute of Applied Biology,College of Life Science,Shanxi University,Taiyuan 030006,China)

机构地区：[1]山西省农业科学院植物保护研究所,农业有害生物综合治理山西省重点试验室,山西太原030031 [2]山西大学生命科学学院,山西大学应用生物学研究所,山西太原030006

出　　处：《山西农业科学》2020年第2期145-147,153,共4页Journal of Shanxi Agricultural Sciences

基　　金：山西省重点研发计划项目（201803D221015-1）;山西省农业科学院特色农业技术攻关项目（YGG17110）;山西省农业科技成果转化和示范推广项目（2017CGZH47）

摘　　要：为了探究长尾仓鼠VKORC1基因与其抗药性的关系,以长尾仓鼠的基因组DNA为模板,使用特异引物对长尾仓鼠的VKORC1基因片段进行扩增并获得其序列。结果表明,使用2对引物可分别扩增出长度为700、600 bp的条带;条带与预测基因片段大小一致,为目标条带。研究应用分子生物学手段探索抗药性监测的新途径,可为进一步获得该基因的全长,并探究VKORC1基因与抗药性的关系奠定基础。To explore the relationship between VKORC1 gene and resistance of Cricetulus longicaudatus, the paper amplified the VKORC1 gene fragment of Cricetulus longicaudatus and obtained its sequence. The VKORC1 gene fragment was amplified with specific primers using the genomic DNA of Cricetulus longicaudatus as template. The result showed that two pairs of primers were used to amplify700, 600 bp bands, respectively. The size of the bands was the same as that of the predicted gene segments, which was the target band. In this study, molecular biological methods were used to explore a new way of resistance monitoring, which laid a foundation for further obtaining the full length of the gene and researching the relationship between VKORC1 gene and resistance.

关 键 词：长尾仓鼠 VKORC1基因 PCR扩增 

分 类 号：S443.3[农业科学—农业昆虫与害虫防治]