REV感染对SPF雏鸡免疫器官细胞凋亡及c-Myc基因表达的影响  

Influence of reticuloendotheliosis virus(REV) infection to cell apoptosis and c-Myc gene expression in immune organs of SPF chicks

作　　者：周放 侯宁[1] 高雪丽[1] 刘超男[1] 张淑君 吴乐 高海婷 边洋 吕晓萍[1] 郑世民[1] ZHOU Fang;HOU Ning;GAO Xue-li;LIU Chao-nan;ZHANG Shu-jun;WU Le;GAO Hai-ting;BIAN Yang;LU Xiao-ping;ZHENG Shi-min(Collage of Veterinary Medicine,Northeast Agricultural University,Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine,Harbin 150030,China)

机构地区：[1]东北农业大学动物医学学院黑龙江省实验动物与比较医学重点实验室

出　　处：《中国兽医科学》2020年第1期34-41,共8页Chinese Veterinary Science

基　　金：国家自然科学基金项目(31472169)

摘　　要：为探究禽网状内皮组织增殖病病毒(REV)感染对雏鸡胸腺、脾和法氏囊细胞凋亡及相关基因c-Myc表达、核质比值的影响,并阐述其相互关系。以1日龄SPF雏鸡为研究对象,应用分子生物学和免疫学技术结合病理学检测方法,通过对上述免疫器官c-Myc基因m RNA及其蛋白含量、c-Myc阳性细胞数、细胞凋亡率及核质比值的检测,较全面系统地研究了REV感染SPF雏鸡后第1、7、14、21、28和42天上述被检指标的动态变化。结果显示,REV感染雏鸡后,无论中枢免疫器官(胸腺、法氏囊)还是外周免疫器官(脾),其c-Myc基因mRNA表达和蛋白含量及阳性细胞数量均不同程度的高于对照雏鸡,其中21~28 d差异显著(P<0.05)或极显著(P<0.01);免疫器官细胞凋亡率在病毒感染后14~28 d极显著(P<0.01)高于对照雏鸡;免疫器官细胞核质比值不同程度的小于对照雏鸡,特别是病毒感染后21~28 d差异显著(P<0.05),以法氏囊细胞凋亡和核质比变化尤为明显;细胞凋亡率和核质比值变化具有较好的相关性(R^2=0.812,P<0.01)。表明REV感染所致免疫器官细胞凋亡与c-Myc基因过表达密切相关,核质比值减小程度可间接反映细胞凋亡程度。This research was conducted to study cell apoptosis,expression of c-Myc gene and nucleoplasm ratio(N/C) in immune organs of specific pathogen-free(SPF) chicks infected with reticuloendotheliosis virus(REV). In this article,1-day-old chicks were used, molecular biology,immunological techniques and pathological detection methods were applied to test the changes of c-Myc gene expression and protein content,number of c-Myc positive cells,apoptotic rate and N/C in immune organs at 1,7,14,21,28 and 42 days.The results showed that in REV infected chicks,the content of c-Myc and the number of c-Myc positive cells in immune organs were significantly higher than control chicks at 21-28 dpi.The apoptotic rate of cells in immune organs was significantly higher than that in control chicks at 14-28 dpi,while N/C was significantly lower than that in control chickens at 21-28 dpi.There was a good correlation between cells apoptotic rate and N/C which suggested that the cells apoptosis in immune organs induced by REV infection is closely related to the overexpression of c-Myc gene,and the reduction of N/C can directly reflect the progress of apoptosis.

关 键 词：网状内皮组织增殖病病毒 SPF雏鸡 免疫器官 C-MYC基因表达 细胞凋亡 

分 类 号：S852.659.3[农业科学—基础兽医学]