清肝利胆口服液化学成分的鉴定及网络药理学法探讨其作用机制  

Identification of the chemical constituents and network pharmacology-based study on mechanisms of Qinggan Lidan mixture

作　　者：张丽珍[1] 李泽运 宋之臻 冯甜甜 朱运静 张晓坚[1] ZHANG Li-zhen;LI Ze-yun;SONG Zhi-zhen;FENG Tian-tian;ZHU Yun-jing;ZHANG Xiao-jian(The First Affiliated Hospital of Zhengzhou University,Henan Zhengzhou 450052,China;Shenyang Pharmaceutical University,Liaoning Shenyang 110015,China)

机构地区：[1]郑州大学第一附属医院,河南郑州450052 [2]沈阳药科大学,辽宁沈阳110015

出　　处：《中国医院药学杂志》2020年第1期38-41,共4页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金资助项目(编号:81603287)

摘　　要：目的:对清肝利胆口服液中的化学成分进行鉴定,探索其保肝利胆的作用机制。方法:采用超高效液相色谱-四级杆-飞行时间质谱(UPLC-Q-TOF-MS/MS)法鉴定清肝利胆口服液中所含的化学成分;利用TCMSP、TTD等数据库获得所得成分和疾病分别相对应的靶点信息;通过生物信息学技术富集涉及的相关通路及生物过程,并运用Cytoscape软件进行构图。结果:鉴定出清肝利胆口服液成分22种,成分作用靶点548个,疾病靶点454个,主要作用通路5条。结论:清肝利胆口服液中化学成分可以通过UPLC-Q-TOF-MS/MS法进行简便、快速定性分析,而其发挥保肝利胆的作用可能与抗炎、免疫调节、细胞色素P450代谢和血糖调节相关。OBJECTIVE To identify the chemical constituents in Qinggan Lidan mixture and explore its potential hepatoprotective mechanism.METHODS UPLC-Q-TOF-MS/MS was performed to identify the chemical constituents in Qinggan Lidan mixture;TCMSP, TTD and other databases were used to obtain the target information corresponding to the obtained components and diseases, respectively;the related pathways and biological processes involved were enriched by bioinformatics techniques and were mapped using Cytoscape software.RESULTS Twenty-two constituents of Qinggan Lidan mixture were identified, which contained 548 effect targets for drug compositions and 454 related targets for disease and 5 main signal pathways.CONCLUSION The chemical constituents in Qinggan Lidan mixture can be analyzed by UPLC-Q-TOF-MS/MS in a simple, rapid and qualitative way, and its role in protecting liver and gallbladder may be related to anti-inflammatory, immune regulation, cytochrome P450 metabolism and blood glucose regulation.

关 键 词：清肝利胆口服液 超高效液相色谱-四级杆-飞行时间质谱 网络药理学 

分 类 号：R917[医药卫生—药物分析学]