密蒙花颗粒剂对去势雄兔泪腺细胞凋亡因子Bax、Caspase-3、Fas和FasL的影响  

Effects of Mimenghua Granules on Apoptosis Factors Bax,Caspase-3,FasL in Lacrimal Gland Cells of Ovariectomized Male Rabbits

作　　者：蒋鹏飞[1,3] 覃艮艳 彭晓芳 张又玮 彭俊 彭清华[1,2,3] JIANFG Peng-fei;QIN Gen-yan;PENG Xiao-fang;ZHANG You-wei;PENG Jun;PENG Qing-hua(Hunan University of Chinese Medicine,Changsha 41020S,China;First Affliated Hospital of Hunan University of Chinese Mediciney Changsha 410007,China;Key Laboratory of Chinese Medicine for Prevention and Treatment of Eye,Ear,Nose and Throat Diseases in Hunan Province,Changsha 410208,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]湖南中医药大学第一附属医院,湖南长沙410007 [3]中医药防治眼耳鼻咽喉疾病湖南省重点实验室,湖南长沙410208

出　　处：《时珍国医国药》2019年第12期2820-2822,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金面上项目(30772824b81574031);中医药防治五官科疾病湖南省重点实验室建设项目(2017TP1018);湖南省长沙市科技计划項目(K1501014-31,kcl704005);中夬财政支持地方高校建设项目;国家中医药管理局中医眼科学重点学科建设项目;湖南省中医五官科学重点学科建设项目。

摘　　要：目的观察密蒙花颗粒剂对去势雄兔泪腺细胞Bax、Caspase-3、Fas和FasL表达的影响:方法30只新西兰长耳白兔随机平均分为5组,分别为:A组:空白组,不灌胃;B组:模型组,不灌胃;C组:密蒙花颗粒剂组,灌胃药物为密蒙花颗粒剂;D组:安慰剂组,灌胃药物为生理盐水;E组:睾酮组,在兔大腿肌肉处注射丙酸睾酮注射液,,除A组外,所有兔子在干预前的造模方式均为双侧睾丸及附睾切除术,并在造模前1天和造模后第30天测定SIT和BUT。干预30天后,将所有兔子处死,摘取泪腺组织进行伊红染色观察泪腺组织结构,免疫组化法检测各组泪腺组织中Bax、Caspase-3、Fas和FasL表达结果HE染色显示:密蒙花颗粒剂组兔子泪腺组织排列整齐,细胞结构清晰,有少量炎症细胞浸润和细胞凋亡,Bax sCaspase-3、Fas和FasL少量表达于细胞膜和细胞浆中免疫组化检测显示密蒙花颗粒剂组Bax、Caspase-3、Fas和FasL平均光密度均较模型组减少(P<0.01).结论密蒙花颗粒剂对去势雄兔泪腺细胞Bax、Caspase-3、Fas和FasL表达具有抑制作用;密蒙花颗粒剂通过抑制泪腺组织中Bax xCaspase-3、Fas和FasL的表达减轻泪腺组织的凋亡,达到治疗干眼的目的。Objective To observe the effects of Mimeng granules on the expression of Bax,Caspase-3,Fas and FasL in ovariec-tomized male rabbit lacrimal gland cells.Methods Thirty New Zealand long-eared white rabbits were randomly divided into 5 groups:group A:blank group,no gavage;group B:model group,no gavage;group C:Mimenghua granule group,gavage drug for the Mimenghua granules;Group D:placebo group,the saline drug was normal saline;Group E:testosterone group,testoster­one propionate injection was injected into the rabbit thigh muscle.Except for group A,all rabbits were treated with bilateral tes­ticular and epididymal resection before intervention,and SIT and BUT were measured 1 day before modeling and 30 days after modeling.After 30 days of intervention,all rabbits were sacrificed.Lacrimal gland tissue was harvested for eosin staining to ob­serve the structure of lacrimal gland.Immunohistochemistry was used to detect tlie expression of Bax,Caspase-3,Fas and FasL in lacrimal gland tissue.Results HE staining showed that the lacrimal gland of rabbits in the Mimenghua granule group was neatly arranged,the cell structure was clear,and there was a small amount of inflammatory cell infiltration and apoptosis.Bax,Caspase-3,Fas and FasL were expressed in the cell membrane and cytoplasm.Immunohistochemistry showed that the average optical densities of Bax,Caspase-3,Fas and FasL in the Mimenghua granule group were lower than those in the model group(P<0.01).Conclusion Mimenghua granules inhibited the expression of Bax,Caspase—3,Fas and FasL in ovariectomized rab­bit lacrimal gland cells;Mimenghua granules alleviated lacrimal gland tissue by inhibiting the expression of Bax,Caspase-3,Fas and FasL in lacrimal gland tissue The apoptosis is achieved for the purpose of treating dry eyes.

关 键 词：密蒙花颗粒剂 去势雄兔 泪腺组织 凋亡因子 

分 类 号：R285.5[医药卫生—中药学]