麦冬硫熏及配伍后指标成分含量变化的近红外光谱技术快速分析  

Rapid analysis of sulfur fumigation of Ophiopogon japonicus and changes of index components after its compatibility based on near infrared spectroscopy

作　　者：席啸虎[1] 王世伟[1] 张小慧[1] XI Xiao-hu;WANG Shi-wei;ZHANG Xiao-hui(Shanxi Provincial Institute of Traditional Chinese Medicine,Taiyuan 030012,China)

机构地区：[1]山西省中医药研究院,山西太原030012

出　　处：《时珍国医国药》2019年第12期2907-2911,共5页Lishizhen Medicine and Materia Medica Research

基　　金：山西省卫生健康委员会科研深题(2017092);山西省中医药大学科技创新能力培育计划项目(2019PY-144,2019PY-156)。

摘　　要：目的运用近红外光谱技术对麦冬硫熏及配伍后成分变化进行快速分析。方法以麦冬so2残留为对照,用相似性判别分析,建立近红外光谱与对照值的定性模型;以麦冬皂苷D(OPD)、D'(OPD'),甲基麦冬二氢高异黄酮A(MA)、B(MB)含量为对照,用偏最小二乘法(PLS)建立近红外光谱与对照值的定量模型。运用HPLC-ELSD同时测足4种成分含量,流动相乙腈-水梯度洗脱,温度60℃,N2流量2.01/min。结果最佳硫熏定性模型:波长4000~10000cm^-1,SNV处理,内、外部验证准确率分别为100%、95%。最佳定量模型:麦冬,SNV,4000~10000cm^-1;麦冬-五味子,Constant,4800~5500,6500~7200cm^-1;麦冬-山药,SNV,4800~5500cm^-1、6500-7200cm^-1;麦冬-黄连,SNV,4000~lOOOOcm^-1。模型相关系数依次为0.9758-0.9845,0.9654-0.9811,0.9821~0.9867,0.9512~0.9768。麦冬-山药配伍指标成分含量增加,麦冬-五味子配伍MA不变、其它增加,麦冬-黄连配伍OPD、OPD'增加,MA、MB减少.结论近红外光谱技术可用于麦冬硫熏定性及指标成分定量快速分析。麦冬不同配伍后指标成分变化情况不同。Objective Near infrared spectroscopy(NIRS)were used to identify the sulfur fumigation and composition changes of Ophiopogon japonicus after compatibility quickly.Methods S02 residue of Ophiopogon japonicus was took as control,a qualitative model between near infrared spectroscopy and control value was established by similarity discriminant analysis.With OPD,OPD;MA and MB as control,a quantitative model between near infrared spectroscopy and control value was established by PLS.Simultaneous determination of four components by HPLC-ELSD was carried out.The mobile phase was acetonitrile-water gradient elution,temperature:60 C,N2 flow rate:2.0 iymin.Results The best qualitative model of sulfur fumigation was:wavelength 4000〜10000cm^-1,SNV treatment,internal and external verification accuracy were 100%and 95%,respectively.Quantitative models were selected as follows:Ophiopogon japonicus,SNV,4000〜10000cm^-1;Ophiopogon japonicus-schisandra,Constant,4800〜5500,6500〜7200cm_1;Ophiopogon japonicus-yam,SNV,4800~5500cm^-1,6500~7200cm^-1;Ophiopogon japonicus-Coptis chinensis,SNV,4000〜10000cm^-1.Content change:After the compatibility of Ophiopogon japonicus and Yam,the content of index components increased.After compatibility of Ophiopogon japonicus and Schisandra chinensis,MA remained unchanged and others increased.OPD and OPDincreased and MA and MB decreased after the combination of Ophiopogon japonicus and Coptis chinensis.Conclusion Near infrared spectroscopy can be used for qualitative detection of sulfur fu mi gat io n in Ophiopogon japonicus and rapid analysis of index components in Ophiopogon japonicus series compatibility.The changes of index components of Ophiopogon japonicus in different compatibility were different.

关 键 词：麦冬 配伍 化学成分 近红外 

分 类 号：R283.1[医药卫生—中药学] R284.2[医药卫生—中医学]