ANXA1拮抗IL-1β导致的胰岛β细胞功能损伤的机制  

ANXA1 Protects IsletβCells against IL-1β-induced Cell Function Damage

作　　者：徐佳亮 孙瞳 陈芳[1] 韩晓[1] XU Jialiang;SUN Tong;CHEN Fang;HAN Xiao(The Key Laboratory of Human Functional Genomics of Jiangsu Province,Nanjing Medical University,Nanjing,211166,China)

机构地区：[1]南京医科大学江苏省人类功能基因组学重点实验室k南京市,211166

出　　处：《医学分子生物学杂志》2020年第1期1-5,共5页Journal of Medical Molecular Biology

基　　金：国家自然科学基金青年项目(81702882);江苏省自然科学基金青年基金项目(No.BK20171056)。

摘　　要：目的探究ANXA1拮抗IL-1β引起的胰岛β细胞功能缺陷的作用及机制.方法将ANXA1基因插入pCMV-HA载体中,构建ANXA1过表达质粒,将其转染入小鼠胰岛β细胞系βtc-6细胞后,用IL-1β处理上述细胞,借助于MTT、葡萄糖刺激的胰岛素分泌实验(GSIS)等方法研究ANXA1对胰岛β细胞的保护作用.结果IL-1β导致胰岛β细胞ANXA1表达降低,同时出现胰岛β细胞存活率下降以及胰岛素合成和分泌量减少;而过表达ANXA1可拮抗上述现象的发生.结论ANXA1保护胰岛β细胞免受IL-1β造成的细胞凋亡增加与功能下降.Objective To explore the protective effect and related mechanism of ANXA1 on IL-1β-induced isletβcell damage.Methods ANXA1 gene was inserted into the pCMV-HA vector to construct an ANXA1 overexpression plasmid.The plasmid was transfected into mouse isletβtc-6 cells,and then,βtc-6 cells were treated with IL-1β.MTT and GSIS experiments were used to ex-plore the protective effect of ANXA1 on isletβcells.Results IL-1βstimulation decreasedβcell survival rate and the expression level of ANXA1 in isletβcells.Overexpression of ANXA1 reversed the decrease in cell survival rate,insulin synthesis and insulin secretion of isletβcells induced by IL-1β.Conclusion ANXA1 has a protective effect on isletβcells.Restoring ANXA1 expression can reverse the functional damage of isletβcells caused by IL-1β.

关 键 词：ANXA1 IL-1Β 胰岛Β细胞 胰岛素分泌实验 

分 类 号：R34[医药卫生—基础医学]