山茱萸提取物对认知障碍大鼠海马神经元的保护作用  

Protective effects of the Cornus officinalis Sieb. et Zucc. extract on hippocampal neurons in rats with cognitive impairment

作　　者：诸葛秀红[1] 韦日明[2] 刘漫君[1] 朱琳 程子娟 曹娜 李肇蕤 李孟知 夏春波[3] ZHUGE Xiu-hong;WEI Ri-ming;LIU Man-jun;ZHU Lin;CHENG Zi-juan;CAO Na;LI Zhao-rui;LI Meng-zhi;XIA Chun-bo(Affiliated hospital of Guilin Medical University,Guilin,Guangxi 541001,China;School of biotechnology,Guilin Medical University,Guilin,Guangxi 541004,China;Department of Anatomy,Guilin Medical University,Guilin,Guangxi 541004,China)

机构地区：[1]桂林医学院附属医院,广西桂林541001 [2]桂林医学院生物技术学院,广西桂林541004 [3]桂林医学院基础医学院,广西桂林541004

出　　处：《南方农业学报》2019年第12期2812-2818,共7页Journal of Southern Agriculture

基　　金：国家自然科学基金项目(81760159,81960163);广西自然科学基金项目(2017GXNSFAA198307,2018GXNSFBA138029)

摘　　要：【目的】明确山茱萸提取物对认知障碍大鼠海马神经元的保护作用及其机制,为开展中药治疗认知障碍的深入研究提供科学依据。【方法】以柠檬酸铝和亚硝酸钠联合腹腔注射60 d建立40只认知障碍大鼠模型,随机分为模型组及山茱萸提取物低、中、高剂量组,分别以4、8和12 g/(kg·d)的山茱萸提取物连续灌胃4周后,利用Morris水迷宫检测各组大鼠的认知功能,采用尼氏染色法观察大鼠海马神经元存活状态,并以TUNEL法检测大鼠海马神经元的凋亡情况。【结果】与模型组相比,山茱萸提取物高、中剂量组大鼠爬上平台所需时间极显著缩短(P<0.01,下同),跨越平台次数极显著增加;山茱萸提取物低剂量组大鼠爬上平台所需时间与模型组间无显著差异(P>0.05),但跨越平台次数显著增加(P<0.05,下同)。山茱萸提取物高、中、低剂量组大鼠海马CA1区神经元尼氏小体数目明显增加,着色加深,对应的平均光密度值分别为0.46±0.11、0.39±0.06和0.32±0.06,均显著高于模型组;山茱萸提取物低、中、高剂量组大鼠海马CA1区绿色荧光标记的神经元数量逐渐减少,凋亡指数逐渐降低,对应的凋亡指数分别为(24.20±3.45)%、(16.33±5.68)%和(9.56±2.80)%,显著或极显著低于模型组[(38.78±4.36)%]。【结论】山茱萸提取物可有效改善认知障碍大鼠在Morris水迷宫中的游泳轨迹,其海马CA1区神经元尼氏小体数目增加、着色加深,绿色荧光标记的神经元逐渐减少,凋亡指数逐渐降低,即山茱萸提取物对大鼠认知功能的保护作用可能与海马CA1区神经元功能改善有关。【Objective】The protective effect of Cornus officinalis Sieb. et Zucc. extract on hippocampal neurons of cognitive impairment rats and its mechanism were studied to provide scientific basis for the further research on the treatment of cognitive impairment with traditional Chinese medicine.【Method】Forty rats with cognitive impairment were produced by intraperitoneal injection of aluminium citrate and sodium nitrite for 60 d and randomly divided into model,lowdose,medium-dose and high-dose groups of C. officinalis extract. The rats of low-dose,medium-dose and high-dose group were respectively given 4,8,12 g/(kg·d)of C. officinalis extract by gavage for 4 weeks. Morris water maze was used to test cognitive function in rats of each group,Nissl staining was used to observe hippocampal neurons survival status and the TUNEL method was used to detect apoptosis of hippocampal neurons in rats.【Result】Compared with the model group,the time of climbing onto the platform of medium-dose and high-dose groups of C. officinalis extract were highly significantly shortened(P<0.01,the same below)and the number of crossing the platform was highly significantly increased. There was no significant difference between the low-dose group and the model group in the time required to climb onto the platform(P>0.05),but the number of crossing the platform increased significantly(P<0.05,the same below). The number of nissl bodies of neurons in the hippocampus CA1 region of the high,medium and low dose groups of C. officinalis extract increased and the staining deepened. The corresponding average optical density values were 0.46±0.11,0.39±0.06 and 0.32±0.06,respectively,which were significantly higher than the model group. The number of green fluorescently labeled neurons in the hippocampal CA1 area of rats in the low,medium and high dose groups of C. officinalis extract decreased gradually,and the apoptosis index decreased gradually. The corresponding apoptosis indexes were(24.20±3.45)%,(16.33±5.68)% and(9.56±2.80)%,which wer

关 键 词：大鼠 山茱萸提取物 认知障碍 海马神经元 尼氏小体 凋亡指数 

分 类 号：S865.12[农业科学—野生动物驯养]