振元复脉颗粒对慢性心律失常模型大鼠的疗效及其部分机制研究  

Study on Efficacy and Part of the Mechanism of Zhenyuan Fumai Granules in Chronic Arrhythmia Model Rats

作　　者：黄玮[1] 张琪[1] 朱月琴[1] 曹琰[1] 刘产明[1] HUANG Wei;ZHANG Qi;ZHU Yueqin;CAO Yan;LIU Chanming(Changzhou Traditional Chinese Medicine Hospital,Changzhou 213003,China)

机构地区：[1]常州市中医医院

出　　处：《世界中医药》2020年第2期235-238,共4页World Chinese Medicine

基　　金：常州市科技计划指导性项目(2017333);常州市孟河医派百人传承培养工程项目

摘　　要：目的:探讨振元复脉颗粒治疗慢性心律失常模型大鼠的可能机制。方法:将60只SD大鼠随机分为空白组(10只)及造模组(50只),造模组大鼠接受尾静脉注射氯化钡溶液制备心律失常模型,将46只成模大叔数字随机分为模型组及干预组,每组23只。其中干预组大鼠利用振元复脉颗粒灌胃,模型组大鼠接受等体积生理盐水灌胃,均干预4周。比较各组大鼠心电图指标,外周血SOD、MDA、心脏组织Na+-K+-ATP、心脏组织PI3K、AKT、p-AKT浓度变化。结果:与模型组比较,振元复脉颗粒可明显缩短模型鼠心律失常持续时间(P<0.05);下降外周血MDA、Bax水平;上调SOD、PI3K、p-Akt、Bcl-2水平及Na+-K+-ATP酶活性,差异有统计学意义(P<0.05)。结论:振元复脉颗粒可明显对抗心率失常,具有保护心肌组织的作用,其作用机制可能与抗氧自由基、抑制心肌细胞凋亡有关。Objective:To explore the possible mechanism of Zhenyuan Fumai Granules in the treatment of chronic arrhythmia model rats.Methods:A total of 60 SD rats were randomly divided into blank group(n=10)and modeling group(n=50).The modeling group was injected with barium chloride solution through tail vein to establish arrhythmia model.A total of 46 model rats were randomly divided into model group(n=23)and intervention group(n=23).Among them, the rats in the intervention group were given intragastric administration of Zhenyuan Fumai Granules for 4 weeks, and the rats in the model group were given intragastric administration of the same volume of normal saline for 4 weeks.ECG indexes, SOD and MDA in peripheral blood, Na+-K+-ATP in heart tissue, as well as concentration changes of PI3 K, AKT and p-AKT in heart tissue were compared in each group.Results:1)Compared with the model group, Zhenyuan Fumai Granules could significantly shorten the duration of arrhythmia(P<0.05), decrease the levels of MDA and Bax in peripheral blood, and increase the levels of SOD, PI3 K, p-Akt and Bcl-2 as well as Na+-K+-ATP activity.The differences were statistically significant(P<0.05).Conclusion:Zhenyuan Fumai Granules can obviously antagonize arrhythmia and protect myocardial tissue, and its mechanism may be related to anti-oxygen free radicals and inhibition of cardiomyocyte apoptosis.

关 键 词：心律失常 振元复脉颗粒 SOD MDA NA+-K+-ATP酶 PI3K-AKT通路 心肌凋亡 

分 类 号：R285.5[医药卫生—中药学]