小麦耐盐相关基因TaRSTR的克隆与功能分析  

Cloning and Functional Analysis of Salt-Tolerant Correlative Gene TaRSTR in Wheat

作　　者：王玉 连娟 王聪 赵利利 赵宝存[1] Wang Yu;Lian Juan;Wang Cong;Zhao Lili;Zhao Baocun(College of Life Sciences,Hebei Normal University,Shijiazhuang 050024,China)

机构地区：[1]河北师范大学生命科学学院

出　　处：《山东农业科学》2020年第1期10-16,共7页Shandong Agricultural Sciences

基　　金：国家转基因生物新品种培育重大专项(2018ZX0800910B);河北师范大学重点科研基金项目(L2018Z08)

摘　　要：高盐是限制植物生长和发育的重要非生物胁迫因子。以耐盐小麦RH8706-49根部基因表达谱芯片结果为基础,利用电子克隆和RT-PCR方法克隆了一个盐胁迫时上调表达的基因,命名为TaRSTR(登录号:EU263918)。荧光定量PCR分析证实该基因受盐胁迫诱导表达,亚细胞定位结果显示TaRSTR蛋白定位在细胞核里。TaRSTR过表达提高了转基因拟南芥在盐胁迫下的种子萌发率及成年植株的耐受性。TaRSTR基因过表达可显著提高已知耐盐相关基因At FRY1、At P5CS1和AtRD29B的表达量,推测TaRSTR基因通过这些标记基因提高了转基因拟南芥的耐盐性。上述结果表明TaRSTR基因过表达能提高植株的耐盐性,是植物耐盐的正调节子。High salinity is a very important abiotic stress factor that inhibits the growth and development of plant. The gene expression profile of salt-tolerant wheat mutant RH8706-49 under salt stress was investigated by microarray chip,and according to the results,a gene induced by salt stress was cloned and named TaRSTR( accession number: EU263918). The real-time PCR analysis results confirmed that TaRSTR was indeed induced by salt stress. TaRSTR was located in nuclear. TaRSTR overexpression improved the seed germination rate and the salt tolerance of adult transgenic Arabidopsis plants under salt stress. TaRSTR overexpression increased the expression levels of abiotic stress related marker genes,such as At FRY1,At P5 CS1 and AtRD29 B. It was predicted that TaRSTR improved the salt tolerance of transgenic Arabidopsis thaliana via these marker genes. In summary,TaRSTR gene was invovled in salt-stress response and could positively regulate the salt tolerance in plant.

关 键 词：小麦 TaRSTR基因 耐盐 实时定量PCR 

分 类 号：S512.103.4[农业科学—作物学] Q781[生物学—分子生物学]