ELISA与TRUST在妊娠合并梅毒患者筛查中的应用价值对比  

Comparison of the application value of ELISA and TRUST in screening for syphilis in pregnant women

作　　者：庄洁伟 郭辉 汪小娟[1] ZHUANG Jiewei;GUO Hui;WANG Xiaojuan(Department of Laboratory,Bao'an People's Hospital,Shenzhen 518100,Guangdong,China)

机构地区：[1]深圳市宝安区人民医院检验科

出　　处：《中国性科学》2019年第12期118-120,共3页Chinese Journal of Human Sexuality

基　　金：深圳市宝安区科技计划基础研究项目(2017JD029)

摘　　要：目的对比酶联免疫吸附试验(ELISA)与甲苯胺红不加热血清试验(TRUST)在妊娠合并梅毒筛查中的应用价值。方法选取2016年3月至2018年9月深圳市宝安区人民医院诊治的208例接受妊娠合并梅毒筛查高危因素孕妇作为研究对象。均行EL ISA与TRUST检测,并以梅毒螺旋体抗体明胶颗粒凝集试验(TPPA)检测为金标准,观察两种方法检测阳性率、检测准确率、检测效能及检测所需费用。结果 ELISA检测阳性率为62.50%,TRUST检测阳性率为52.40%,差异具有统计学意义(P<0.05);以TPPA检测为金标准,ELISA检测准确率为98.48%,TRUST检测准确率为82.58%,差异具有统计学意义(P<0.05);ELISA检测敏感性、特异性、阳性预测值、阴性预测值分别为90.91%、98.68%、99.23%、96.15%,ELISA检测上述分别为78.03%、90.79%、94.50%、69.70%,差异均具有统计学意义(P<0.05);ELISA检测所需费用与TRUST检测所需费用对比,明显较少,差异具有统计学意义(P<0.05)。结论妊娠合并梅毒患者筛查中,ELISA检测准确度及检测效能均明显优于TRUST,且ELISA检测经济性更好,更值得推广。Objective To compare the application value of enzyme-linked immunosorbent assay(ELISA) and toluidine red unheated serum test(TRUST) in the screening for syphilis in pregnant women. Methods 208 pregnant women receiving screening for high risk factors of syphilis during pregnancy from March 2016 to September 2018 were selected as the study subjects. All patients were tested by ELISA and TRUST, and the detection of Treponema pallidum antibody gelatin particle agglutination test(TPPA) was the gold standard. The positive rate, detection accuracy, detection efficiency and detection cost of the two methods were observed. Results The positive rates of ELISA and TRUST were respectively 62.50% and 52.40%, with statistically significant difference(P<0.05). With TPPA as the gold standard, the detection accuracy of ELISA and TRUST was 98.48% and 82.58% respectively, with statistically significant difference(P<0.05). The sensitivity, specificity, positive predictive value and negative predictive value of ELISA detection were respectively 90.91%, 98.68%, 99.23% and 96.15%, but the above-mentioned values of ELISA detection were respectively 78.03%, 90.79%, 94.50% and 69.70%, all with statistically significant differences(P<0.05). Compared with the cost of TRUST detection, the cost of ELISA detection was significantly lower, with statistically significant difference(P<0.05). Conclusions In the screening for syphilis in pregnant women, the accuracy and efficiency of ELISA detection is significantly better than that of TRUST detection with lower cost, which is worthy of promotion.

关 键 词：妊娠 梅毒 血清学检测 酶联免疫吸附试验 甲苯胺红不加热血清试验 

分 类 号：R759[医药卫生—皮肤病学与性病学]