鹿茸角柄断面伤口无疤痕愈合过程中骨形态蛋白的表达及功能分析  

Functional research and expression analysis of BMPs during the scarless wound healing process of pedicle stump

作　　者：林珊珊 郭倩倩[2] 陈奕其 郑慧洁 郑美霞 褚文辉 LIN Shanshan;GUO Qianqian;CHEN Yiqi;ZHENG Huijie;ZHENG Meixia;CHU Wenhui(School of Life Science,Taizhou University,Taizhou 318000,China;Institute of Special Animal and Plant Sciences,State Key Laboratory for Molecular Biology of Special Economic Animals,Deer Antler En-gineering Research Center of Jilin Province,Chinese Academy of Agricultural Sciences,Changchun 130012,China)

机构地区：[1]台州学院生命科学学院,台州318000 [2]中国农业科学院特产研究所,特种经济动物分子生物学国家重点实验室,吉林省鹿茸工程研究中心,长春130012

出　　处：《兽类学报》2020年第1期47-53,共7页Acta Theriologica Sinica

基　　金：吉林省自然科学基金(20170101032JC);吉林省自然科学基金(20170101003JC);台州学院校立项目(QD2018142);吉林省科技发展计划项目(20170307007YY);中国农业科学院中央级公益性科研院所基本科研业务费专项(1610342016001,1610342017001,1610342018003)

摘　　要：为研究骨形态蛋白(bone morphogenetic proteins,BMP)在鹿茸再生早期角柄断面伤口无疤痕愈合过程中的功能,本研究通过免疫组化技术对比分析了BMP 2和BMP 4在正常皮肤及茸皮中的表达及分布差异,同时利用添加外源性蛋白分析了BMP 4对鹿真皮成纤维细胞和毛乳头细胞的影响。结果显示:(1)原代培养的真皮成纤维细胞表达波形蛋白阳性率几乎为100%;(2)BMP 2和BMP 4强烈表达于茸皮中新生毛囊的毛基质细胞中;(3)BMP 4可促进鹿真皮成纤维细胞向脂肪细胞转分化;(4)BMP 4可促进鹿真皮毛乳头细胞成团。以此推测BMP在鹿毛囊形成及伤口愈合过程中发挥重要作用。The aim of this study is to find out the functional roles of bone morphogenetic proteins(BMP)during the scar-less wound healing of pedicle stump at the early regeneration stage of antler.Immunohistochemistry(IHC)was used to ana-lyze the expression and localization of BMP 2 and BMP 4 in the pedicle and the antler skins.BMP 4 was added to the cul-ture medium of dermal papilla cells and fibroblasts of deer skin to see if the protein was playing an essential role during trans-differentiation and gathering.The results showed that:(1)Nearly 100%of the fibroblasts harvested from the primary culture of pedicle skin tissue expressed vimentin:a marker of dermal fibroblasts;(2)BMP 2 and BMP 4 were strongly expressed in the newly formed hair matrix cells in the antler skin;(3)BMP 4 promoted the trans-differentiation of fibro-blasts into adipocytes;(4)BMP 4 also facilitated the degree of agglomeration of dermal papilla cells in vitro.Based on these observations we speculated that BMPs played an important role in the process of trans-differentiation of fibroblast and gathering of papilla cells which could accelerate the process of scarless wound healing.

关 键 词：鹿茸 骨形态蛋白 角柄 无疤痕愈合 毛囊 转分化 

分 类 号：Q344[生物学—遗传学]