中药软肝饮对HBV转基因小鼠肝脏保护作用的实验研究  

Experimental study on the protective offect of Chinese medicine Ruanganyin on the liver of HBV transgenic mice

作　　者：张先姚 张国梁[2] 侯勇[2] 刘丽丽[2] 李艳[2] 施美 韩啥 周灏 李梦梦 王姣 ZHANG Xian-yao;ZHANG Guo-liang;HOU Yong;LIU Li-li;LI Yan;SHI Mei;HAN Sha;ZHOU Hao;LI Meng-meng;WANG Jiao(Anhui University of Chinese Medicine, Hefei, 230031;The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, 230031)

机构地区：[1]安徽中医药大学 [2]安徽中医药大学第一附属医院,安徽合肥230031

出　　处：《海南医学院学报》2020年第3期161-164,共4页Journal of Hainan Medical University

基　　金：国家自然科学基金项目(81874451)~~

摘　　要：目的:探讨中药软肝饮对HBV转基因小鼠肝脏的保护作用。方法:通过将50只雌雄各半HBV转基因小鼠随机分为治疗组(高、中、低剂量软肝饮)、模型组、阳性对照组(恩替卡韦分散片)以及10只非HBV转基因小鼠作为空白对照,药物干预灌胃4周后处死小鼠,观察各组小鼠血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、总胆红素(TBIL)水平以及层黏连蛋白(LN)、透明质酸(HA)、Ⅳ型胶原酶(Ⅳ-C)、Ⅲ型前胶原(PCⅢ)水平的表达。结果:(1)与空白对照组相比,模型组ALT、AST、TBIL水平明显比空白对照组高,差异具有统计学意义(P<0.01);与模型组相比,治疗后软肝饮高剂量组ALT、AST、TBIL水平明显低于模型组,差异有统计学意义(P<0.05);治疗后阳性对照组ALT、AST、TBIL水平低于模型组,差异有统计学意义(P<0.05);而治疗后软肝饮中剂量、低剂量组ALT、AST、TBIL水平与模型组比较,差异无统计学意义(P>0.05)。(2)与空白对照组相比,模型组LN、HA、Ⅳ-C、PCⅢ水平明显比空白对照组高,差异具有统计学意义(P<0.01);与模型组相比,治疗后软肝饮高剂量组和阳性对照组LN、HA、Ⅳ-C水平明显低于模型组,差异具有统计学意义(P<0.01);治疗后软肝饮高剂量组和阳性对照组PCⅢ水平低于模型组,差异具有统计学意义(P<0.05);而治疗后软肝饮中剂量、低剂量组LN、HA、Ⅳ-C、PCⅢ水平与模型组比较,差异无统计学意义(P>0.05)。结论:中药复方软肝饮具有保肝降酶、抗肝纤维化的作用,但其抗肝纤维化的机制还需进一步实验进行探讨研究。Objective To study the protective effect of Ruanganyin on the liver of HBV transgenic mice.Methods A total of 50 half HBV transgenic mice,male and female,were randomly divided into treatment group(high,medium and low dose Ruanganyin),model group,positive control group(entecavir dispersible tablet)and 10 non HBV transgenic mice as blank control.The mice were killed after 4 weeks of drug intervention.The serum levels of ALT,AST,TBIL,LN,HA,Ⅳ-C and PCⅢwere observed.Results(1)Compared with the blank control group,the ALT,AST and TBIL levels in the model group were significantly higher than those in the blank control group(P<0.01);compared with the model group,the ALT,AST and TBIL levels in the high-dose group of ruanganyin after treatment were significantly lower than those in the model group(P<0.05);the ALT,AST and TBIL levels in the positive control group were lower than those in the model group(P<0.01/P<0.05).There was no significant difference in ALT,AST and TBIL levels between the two groups(P>0.05).(2)Compared with the blank control group,the level of LN,HA,Ⅳ-C and PCⅢin the model group was significantly higher than that in the blank control group(P<0.01);compared with the model group,the level of LN,HA andⅣ-C in the high-dose group and the positive control group after treatment was significantly lower than that in the model group(P<0.01);the level of PCⅢin the high-dose group and the positive control group after treatment was significantly lower than that in the model group(P<0.01);There was no significant difference in LN,HA,Ⅳ-C and PCⅢlevels between the two groups(P>0.05).Conclusion Chinese medicine Ruanganyin has the effects of protecting liver,reducing enzyme and anti fibrosis,but its mechanism of anti fibrosis needs further study.

关 键 词：HBV转基因小鼠 软肝饮 肝功生化指标 肝纤维化 肝纤四项指标 

分 类 号：R285.5[医药卫生—中药学]