lncRNA SNHG3在慢性阻塞性肺疾病患者外周血单个核细胞表达与临床意义  

Expression and clinical significance of lncRNA SNHG3 in peripheral blood mononuclear cells of patients with chronic obstructive pulmonary disease

作　　者：徐建光[1] 赵洁 杨益秀 陈飞 黄良 李秀宽 郭多见 孙娟 周小曼[3,4] 柳建芳 冯琼[2] 石慧芳 丁毅鹏 XU Jian-guang;ZHAO Jie;YANG Yi-xiu;CHEN Fei;HUANG Liang;LI Xiu-kuan;GUO Duo-jian;SUN Juan;ZHOU Xiao-man;LIU Jian-fang;FENG Qiong;SHI Hui-fang;DING Yi-peng(Department of Respiratory Medicine, the Second Affiliated Hospital of Hainan Medical University, Haikou 570311, Hainan, China;Hainan Hospital Affiliated to Nanhua University, Haikou 570311, Hainan, China;Department of General Medicine, Hainan Hospital Affiliated to Hainan Medical University, Haikou 570311, Hainan, China;Department of General Medicine, Hainan General Hospital, Haikou 570311, Hainan, China;Nanyang Branch of Health Center of Wencheng Town, Wenchang City, Hainan, China;Xincun Health Center of Lingshui Li Autonomous County, Hainan, China;Meitai Health Center of Lingao County, Hainan, China;Zuguan Health Center of Lingshui Li Autonomous County, Hainan, China)

机构地区：[1]海南医学院第二附属医院呼吸内科,海南海口570311 [2]南华大学附属海南医院,海南海口570311 [3]海南医学院附属海南医院全科医学科,海南海口570311 [4]海南省人民医院全科医学科,海南海口570311 [5]文昌市文城镇卫生院南阳分院,海南文昌571399 [6]陵水黎族自治县新村中心卫生院,海南陵水572429 [7]临高县美台卫生院,海南临高571824 [8]陵水黎族自治县祖关卫生院,海南陵水572434

出　　处：《海南医学院学报》2020年第2期107-110,116,共5页Journal of Hainan Medical University

基　　金：国家自然科学基金资助项目(81160008和81660013)~~

摘　　要：目的研究lncRNA SNHG3在慢性阻塞性肺疾病(COPD)患者外周血单个核细胞表达,探讨其与慢性阻塞性肺疾病临床意义。方法收集120例COPD病人与110例正常人对照单个核细胞,RT-PCR方法检测外周血单个核细胞中lncRNA SNHG3的表达量并统计分析COPD组与正常组表达差异,COPD肺功能轻度、中度、重度与极重度组之间的表达差异。结果COPD组外周血单个核细胞中SNHG3相对表达为(1.52±0.52),低于正常对照组(2.51±0.59)(P<0.001)。肺功能轻度组外周血单个核细胞中SNHG3相对表达为(2.16±0.23),高于中度组(1.55±0.10)(P<0.001)、重度组(1.19±0.11)(P<0.001)、极重度组(0.89±0.06)(P<0.001)。此外,lncRNA SNHG3可以靶向结合并调控miR-186的表达。结论慢性阻塞性肺疾病患者外周血单个核细胞中lncRNA SNHG3表达量低可能是COPD的危险因素,这或许能够作为COPD患者严重程度的指标之一。lncRNA SNHG3/miR-186调控网络可能在COPD的发生发展过程中起到重要作用。Objective To investigate the expression of lncRNA SNHG3 in peripheral blood mononuclear cells of patients with chronic obstructive pulmonary disease(COPD),and its clinical significance in COPD.MethodsMononuclear cells were collected from 120 patients with COPD and 110 normal controls.The expression of lncRNA SNHG3 in peripheral blood mononuclear cells was detected by RT-PCR and the difference between COPD group and normal group,as well as between mild,moderate,severe,extremely severe COPD and health volunteers was analyzed.ResultsThe relative expression of SNHG3 in peripheral blood mononuclear cells of COPD patients was(1.52±0.52),which was lower than that of the normal control group(2.51±0.59)(P<0.001).The relative expression of SNHG3 in peripheral blood mononuclear cells of mild COPD was(2.16±0.23),higher than that of moderate group(1.55±0.10)(P<0.001),severe group(1.19±0.11)(P<0.001),and extremely severe group(0.89±0.06)(P<0.001).In addition,lncRNA SNHG3 could bind to miR-186 and regulate its expression.Conclusions The low expression of lncRNA SNHG3 in peripheral blood mononuclear cells of COPD patients may be a risk factor for COPD and an indicator of the severity of COPD patients.The lncRNA SNHG3/miR-186 regulatory network may play an important role in the development of COPD.

关 键 词：COPD SNHG3 单个核细胞 临床指标 

分 类 号：R563.9[医药卫生—呼吸系统]