IL-34诱导肺成纤维细胞IL-6、IL-8表达机制研究  

Molecular mechanism of IL-34 induces expression of IL-6 and IL-8 in lung fibroblasts

作　　者：吴夏楠 罗艳[1] 邹鳞 赖晓霏[1] WU Xia-Nan;LUO Yan;ZOU Lin;LAI Xiao-Fei(Department of Clinical Laboratory,the First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China)

机构地区：[1]重庆医科大学附属第一医院医学检验科

出　　处：《中国免疫学杂志》2020年第4期405-410,共6页Chinese Journal of Immunology

摘　　要：目的:探究IL-34诱导肺成纤维细胞IL-6、IL-8表达的分子机制。方法:体外培养原代人肺成纤维细胞,给予外源性重组IL-34刺激,检测IL-6、IL-8 mRNA和蛋白的变化及MAPK、PI3K-Akt、JAK等信号通路的活化;MAPK、PI3K-Akt、JAK等信号分子抑制剂预处理细胞,观察IL-34刺激IL-6、IL-8 mRNA和蛋白的变化。结果:IL-34可以诱导肺成纤维细胞中IL-6、IL-8表达上调及MAPK、PI3K-Akt、JAK、NF-κB信号通路的活化;特异性信号分子抑制剂可以逆转IL-34诱导肺成纤维细胞中IL-6、IL-8 mRNA和蛋白表达的升高。结论:IL-34可以诱导肺成纤维细胞中IL-6、IL-8表达上调,MAPK、PI3K-Akt、JAK、NF-κB等多种信号通路参与了IL-34诱导IL-6、IL-8表达的信号转导过程。Objective:To explore the molecular mechanism of IL-34 inducing the expression of IL-6 and IL-8 in pulmonary fibroblasts.Methods: Primary human lung fibroblasts were cultured and stimulated by exogenous recombinant IL-34.Changes of IL-6,IL-8 mRNA and protein as well as activation of MAPK,PI3K-Akt,JAK,NF-κB signaling pathways were detected.Lung fibroblasts were preprocessed with MAPK,PI3K-Akt,JAK,NF-κB signal molecular inhibitors,the expression of IL-6,IL-8 mRNA and protein were detected upon IL-34 stimulation.Results: IL-34 induced up-regulation of IL-6 and IL-8 expression by activating of MAPK,PI3K-Akt,JAK,NF-κB signaling pathway as dose and time-dependant manner in lung fibroblasts.Specific signaling molecule inhibitors can reverse the induction of IL-34 on IL-6 and IL-8 expression.Conclusion: IL-34 induces IL-6 and IL-8 expression in lung fibroblasts.Multiple signaling pathways such as MAPK,PI3K-Akt,JAK,and NF-κB are involved in the induction of IL-34 on IL-6 and IL-8 expression.

关 键 词：IL-34 炎症 肺成纤维细胞 

分 类 号：R392[医药卫生—免疫学]