Modified stepwise mini-incision microdissection testicular sperm extraction:a useful technique for patients with a history of orchidopexy affected by non-obstructive azoospermia  

分步法小切口改良睾丸显微取精术在隐睾下降固定术后非梗阻性无精子症的临床应用研究（英文）

作　　者：Peng LI Chen-cheng YAO Er-lei ZHI Yuan XU Zhong WAN Ying-chuan JIANG Yu-hua HUANG Yue-hua GONG Hui-xing CHEN Ru-hui TIAN Chao YANG Liang-yu ZHAO Zheng LI 

机构地区：[1]Department of Andrology,Center for Men’s Health,Urologic Medical Center,Shanghai General Hospital,Shanghai Jiao Tong University,Shanghai 200080,China [2]Department of Urology,Shanghai General Hospital of Nanjing Medical University,Shanghai 200080,China [3]Reproductive Medicine Center,Shanghai General Hospital,Shanghai Jiao Tong University,Shanghai 200080,China [4]Department of Urology,Zhoushan Hospital,Zhoushan 316021,China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2020年第1期87-92,共6页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：Project supported by the National Natural Science Foundation of China(Nos.81701524,81671512,and 81701428);the Frontier Technology Project of Shanghai(No.SHDC12015122),China

摘　　要：Non-obstructive azoospermia(NOA),which is defined as the absence of spermatozoa in the ejaculate secondary to impaired spermatogenesis within the testis,may be caused by a variety of etiologies,including varicocele-induced testicular damage,cryptorchidism,prior testicular torsion,post-pubertal mumps orchitis,gonadotoxic effects from medications,genetic abnormalities,chemotherapy/radiation,and other unknown causes currently classified as idiopathic(Cocuzza et al.,2013).The microdissection testicular sperm extraction(micro-TESE)technique involves a meticulous microsurgical exploration of the testicular parenchyma to identify and selectively extract larger seminiferous tubules that carry a higher probability of complete spermatogenesis(Schlegel,1999).目的:探讨分步法小切口改良睾丸显微取精术在隐睾下降固定术后非梗阻性无精子症的有效性。创新点:既往有隐睾下降固定术手术史的非梗阻性无精子症的患者睾丸局部粘连严重,所以睾丸显微取精术时睾丸暴露困难,因此手术难度明显增大。术者将常规睾丸显微取精术改良为分步法小切口睾丸显微取精术,术中无需完全游离暴露睾丸,仅通过小切口睾丸显微取精术就可使一半的患者成功获取精子。因此分步法小切口改良睾丸显微取精术可在不降低睾丸取精成功率的前提下缩短手术时间和减少手术创伤。方法:收集2015年5月至2017年3月于上海交通大学附属第一人民医院泌尿中心男科接受睾丸显微取精术的20例既往有隐睾下降固定术手术史的非梗阻性无精子症,采用分步法小切口改良睾丸显微取精术获取睾丸精子。分步法小切口改良睾丸显微取精术具体步骤为:第一步,小切口睾丸取精术;第二步,将小切口延长为标准切口,行标准睾丸显微取精术;第三步,对侧睾丸标准睾丸显微取精术。结论:小切口睾丸显微取精术可快速有效获取睾丸内表浅位置饱满的生精小管,标准的睾丸显微取精术可作为小切口睾丸显微取精术失败时的补救措施。分步法小切口睾丸显微取精术可降低睾丸损伤前提下,快速有效地获取睾丸精子。这种手术方式特别适用于睾丸内存在表浅饱满生精小管的非梗阻性无精子症,如隐睾下降固定术后的非梗阻性无精子症。

关 键 词：非梗阻性无精子症 隐睾 睾丸显微取精术 睾丸下降固定术 精子获取 

分 类 号：R699.8[医药卫生—泌尿科学]