胞质Ca2+参与外源H2S促进盐碱胁迫下裸燕麦种子萌发  

Cytosolic Ca2+ Involved in Seed Germination of Naked Oat Enhanced by Exogenous H2S under Saline-Alkali Stress

作　　者：刘建新[1] 欧晓彬 王金成[1] LIU Jian-Xin;OU Xiao-Bin;WANG Jin-Cheng(University Provincial Key Laboratory for Protection and Utilization of Longdong Bio-resources in Gansu Province/College of Life Sciences and Technology,Longdong University,Qingyang 745000)

机构地区：[1]甘肃省高校陇东生物资源保护与利用省级重点实验室/陇东学院生命科学与技术学院

出　　处：《植物研究》2020年第1期58-65,共8页Bulletin of Botanical Research

基　　金：国家自然科学基金(31560125; 31960375);甘肃省庆阳市科技计划项目(KZ2014-19)~~

摘　　要：硫化氢(Hydrogen sulfide,H2S)是植物新型气体信号分子,钙离子(Calcium,Ca2+)为重要的第二信使,两者在植物逆境响应中分别发挥着重要作用。为明确胞质Ca 2+在外源H 2S促进盐碱胁迫下作物种子萌发中的作用,以裸燕麦(Avena nude)为材料,采用培养皿培养,以混合盐碱(NaCl、Na2SO4、Na2CO3、NaHCO3的摩尔比为12∶[KG-*2/3]8∶[KG-*2/3]1∶[KG-*2/3]9)模拟甘肃裸燕麦种植地盐碱环境,蒸馏水为对照,测定了胞外Ca2+螯合剂乙二醇-双-(2-氨基乙醚)四乙酸(EGTA)、质膜Ca2+通道阻断剂氯化镧(LaCl3)、液泡Ca2+释放抑制剂钌红(RR)和内质网钙泵阻断剂毒胡萝卜素(Thaps)分别与H 2S供体硫氢化钠(NaHS)共处理下种子的发芽势、发芽率、发芽指数、活力指数、平均发芽速率、胚根长和胚芽长7个发芽指标,利用隶属函数分析方法综合评价胞质Ca2+对H2S缓解盐碱胁迫抑制种子萌发的影响。结果表明,随着盐碱胁迫浓度增大,裸燕麦种子的发芽势、发芽率、发芽指数、活力指数、平均发芽速率、胚根长和胚芽长显著下降。与对照相比,15~75 mmol·L-1盐碱胁迫导致裸燕麦种子萌发的隶属函数综合评价值(D)显著降低,30mmol·L-1盐碱胁迫下D值下降了73.1%;100~1000μmol·L-1 NaHS不同程度提高了裸燕麦种子萌发的D值,且100μmol·L-1 NaHS缓解30mmol·L-1盐碱胁迫下D值下降的作用最大;EGTA、LaCl3和RR均显著逆转了100μmol·L-1 NaHS对30 mmol·L-1盐碱胁迫下D值下降的缓解作用,而Thaps对NaHS的作用无显著影响。表明胞质Ca2+参与外源H 2S促进盐碱胁迫下裸燕麦种子萌发的信号传导过程,且胞质Ca2+主要来源于胞外Ca2+的内流和液泡中Ca2+的释放。Hydrogen sulfide(H2S)is a new gas signal molecule and calcium(Ca2+)is an important second messenger.Both play important roles in plant adversity response,respectively.The experiment was conducted to clarify the role of cytosolic Ca2+in germination of crop seed promoted by exogenous H2S under saline-alkali stress.Naked oat(Avena nude)was selected as experimental material which was cultured in petri dishes in incubator.Mixed saline-alkaline solution(NaCl∶[KG-*2/3]Na2SO4∶[KG-*2/3]Na2CO3∶[KG-*2/3]NaHCO3 with the mole ratio of 12∶[KG-*2/3]8∶[KG-*2/3]1∶[KG-*2/3]9)was used to simulate the saline-alkaline stress of naked oat growing areas in Gansu Province,extracellular Ca2+chelator ethylene glycol-bis-(2-aminoethylether)-N,N,N′,N′-tetraacetic acid(EGTA),plasma membrane Ca2+channel blocker lanthanum chloride(LaCl3),tonoplast Ca 2+release inhibitor ruthenium red(RR),and endoplasmic reticulum calcium pump blocker thapsigargin(Thaps)were combined with sodium hydrosulfide(NaHS,a donor of H 2S),respectively,and distilled water treatments were set as control.Seven germination indexes including germination potential,germination rate,germination index,vigor index,average germination speeds,radicle length,and plantule length of naked oat under control,saline-alkaline stress and co-treatments were determined.The membership function analysis was performed to comprehensively evaluate the effect of cytosolic Ca 2+on seed germination of naked oat enhanced by H 2S under saline-alkali stress.The germination potential,germination rate,germination index,vigor index,average germination rate,radicle length and plantule length of naked oat seeds decreased significantly with the increase of saline-alkaline concentration.Compared with the control,the 15-75 mmol·L-1 salt-alkaline stresses resulted in a significant decrease in the membership function comprehensive evaluation value D,D value decreased by 73.1%under 30[JP3]mmol·L-1 saline-alkali stress.The 100-1000μmol·L-1 NaHS increased the D value to varying degrees,and 100μ

关 键 词：硫化氢 CA2+ 裸燕麦 盐碱胁迫 种子萌发 

分 类 号：S543+603.4[农业科学—作物学] Q945.78[生物学—植物学]