超声辅助酸水解提取山里红叶中牡荆素的工艺优化  

Optimization of Ultrasound Assisted Acid Hydrolysis Process for Extracting Vitexin from Crataegus pinnatifida Bge. var. major N. E. Br. Leaf

作　　者：邢文淼 陈民 冯昌寅 孟宪明 田梦飞 牟璠松[1] 罗猛[1] XING Wen-Miao;CHEN Min;FENG Chang-Yin;MENG Xian-Ming;TIAN Meng-Fei;MU Fan-Song;LUO Meng(College of Chemistry,Chemical Engineering and Resource Utilization,Northeast Forestry University,Harbin 150040)

机构地区：[1]东北林业大学化学化工与资源利用学院

出　　处：《植物研究》2020年第1期148-152,共5页Bulletin of Botanical Research

基　　金：国家重点研发计划项目(2017YFD0600605);黑龙江省博士后科研启动金(LBH-Q13003);中央高校基本科研业务费专项资金项目(2572014CA08)~~

摘　　要：采用正交法优化山里红叶中牡荆素的超声辅助提取工艺。通过盐酸浓度、提取时间、固液比、超声功率、超声温度、乙醇浓度6种影响因素的单因素实验,研究它们与牡荆素提取得率之间的规律趋势;应用正交设计优化试验,研究盐酸浓度、提取时间、固液比和超声功率对山里红叶提取牡荆素得率影响大小,并确定最佳提取工艺。研究结果表明:当盐酸浓度为2 mol·L-1、提取时间为40 min,固液比为1∶[KG-*2/3]20、超声功率为500 W、超声温度为50℃、乙醇浓度为50%时,效果最佳,得率为2.603 mg·g-1;盐酸浓度具有较大显著性,且各因素影响顺序为:盐酸浓度>超声功率>提取时间>固液比。Ultrasound-assisted extraction of vitexin from Crataegus pinnatifida Bge.var.major N.E.Br.leaf was optimized by orthogonal design.Through the single factor experiments of six factors the HCl content,extraction time,solid-liquid ratio,ultrasonic power,ultrasonic temperature and ethanol concentration,the regularity trend between them and the vitexin extraction yield was studied.[JP3]The optimization experiment with orthogonal design was conduted to study the effects of HCl content,extraction time,solid-liquid ratio and ultrasonic power on the extraction yield of C.pinnatifida Bge.var.major N.E.Br.and the optimal extraction process were determined.When the HCl content was 2 mol·L-1,the extraction time was 40 min,the solid-liquid ratio was 1∶[KG-*2/3]20,the ultrasonic power was 500 W,the ultrasonic temperature was 50℃,and the ethanol concentration was 50%,the best result was obtained and the yield was 2.603 mg·g-1.The HCl content was significant,and the order of influence of each factor was:[JP]HCl concentration>ultrasonic power>extraction time>solid-liquid ratio.

关 键 词：山里红叶 牡荆素 提取工艺 超声辅助 正交试验 

分 类 号：Q949.95[生物学—植物学]