黄连-白术复方对小鼠胃溃疡模型的预防作用  

Preventive effects of Rhizoma Coptidis and Atractylodes on mice with gastric-ulcer

作　　者：尤思路[1] 李娟 晏陶 彭芳 刘沙 YOU Silu;LI Juan;YAN Tao;PENG Fang;LIU Sha(School of Pharmacy,Chengdu Medical College,Chengdu 610500,China)

机构地区：[1]成都医学院药学院

出　　处：《中南大学学报（医学版）》2020年第1期8-16,共9页Journal of Central South University ：Medical Science

基　　金：国家自然科学基金(81803514);四川省教育厅科研基金(17ZB0137);成都医学院科研基金(CYZ16-18)~~

摘　　要：目的:研究黄连和白术的配伍对小鼠胃溃疡模型的预防作用。方法:将小鼠随机分为正常组、胃溃疡模型对照组、雷尼替丁阳性药物组、黄连组、白术组、黄连-白术药对不同配比(黄连:白术为9꞉1,8꞉2,7꞉3,6꞉4,5꞉5,4꞉6)组。其中黄连组、白术组、黄连-白术药对配比组按照给药剂量的不同又分别设置高剂量组、中剂量组和低剂量组。通过对小鼠预防性灌药6 d(正常组和胃溃疡模型对照组给予等量生理盐水)后灌胃无水乙醇(正常组除外)建立胃溃疡模型。处死小鼠后取全胃剖开,肉眼观察胃黏膜形态,计算不同处理组溃疡抑制率,确定最佳药材配比与剂量。在显微镜下观察胃组织病理学变化,并检测血清超氧化物歧化酶(superoxide dismutase,SOD),丙二醛(malondialdehyde,MDA),一氧化氮(nitric oxide,NO),内皮素-1(endothelin-1,ET-1)含量,验证最佳配比组抗溃疡效果。结果:黄连-白术复方能显著降低无水乙醇致胃小鼠溃疡模型溃疡面积(P<0.001),且配伍后的药效大多明显优于单用黄连和白术的药效(P<0.05,P<0.01或P<0.001)。黄连꞉白术为6꞉4配比组抗溃疡效果最显著,且该配比下高剂量组抗胃溃疡药效明显优于雷尼替丁阳性药物组(P<0.001)。雷尼替丁阳性药物组、黄连高剂量组、白术高剂量组、黄连+白术(6꞉4)高剂量组均能显著降低溃疡模型小鼠血清中MDA和ET-1含量(P<0.01或P<0.001),提高SOD和NO含量(P<0.01或P<0.001)。结论:黄连-白术复方对无水乙醇法致小鼠胃溃疡具有预防作用,该作用表现出配比依赖和剂量依赖的趋势,其机制可能与黄连和白术的抗氧化、舒张血管等作用有关。二者联合用药表现出协同作用。Objective:To detemine preventive effects of compound formula Rhizoma Coptidis and Atractylodes on mice with gastric-ulcer.Methods:The mice were randomly divided into a normal group,a gastric ulcer group,a ranitidine positive drug group,a Rhizoma Coptidis group,an Atractylodes group,and a Rhizoma Coptidis plus Atractylodes group(the ratios of Coptidis to Atractylodes were 9꞉1,8꞉2,7꞉3,6꞉4,5꞉5,or 4꞉6,respectively).Gastric ulcer models were established by intragastric administration of anhydrous ethanol after 6 days of preventive infusion.The mice were killed 6 days after the treatments.The whole stomach was opened to observe gross morphology of gastric mucosa.The pathological changes of gastric tissue were observed under microscope,and serum samples were collected to detect the contents of superoxide dimutase(SOD),malondialdehyde(MDA),NO,and endothelin-1(ET-1).Results:The Rhizoma Coptidis and Atractylodes decoction significantly decreased ulcer area(P<0.001),and the effects of compound formula are better than those of Coptidis and Atractylodes alone(P<0.05,P<0.01,or P<0.001).The anti-ulcer effect of compound formula(Coptidis꞉Atractylodes=6꞉4)was the best one,and the anti-gastric ulcer effect of the high-dose group was significantly better than that of the ranitidine-positive group(P<0.001).The ranitidine positive drug group,the high-dose group of Rhizoma Coptidis,the high-dose group of Atractylodes,and the high-dose group of Rhizoma Coptidis-Atractylodes(6꞉4)significantly reduced MDA,ET-1(P<0.01 or P<0.001),and significantly increased SOD,NO in serum(P<0.01 or P<0.001).Conclusion:Rhizoma Coptidis and Atractylodes decoction exerts the effect on preventing ethanol-induced gastric ulcer in mice in a ratio-dependent and dose-dependent manner.The mechanism might be related to anti-oxidation and relaxion of blood vessels.The combination of the two drugs shows a synergistic effect.

关 键 词：中药配伍 预防作用 胃溃疡 灌胃 溃疡抑制率 

分 类 号：R285.5[医药卫生—中药学]