HBV前C区及基因启动子区基因变异与乙肝肝硬化相关性初步研究  

Preliminary analysis of correlation between gene mutation in pre-core and basic core promoter of hepatitis B virus and hepatitis B-induced liver cirrhosis

作　　者：魏芳芳 李文莉[1] 甄丽敏 廖常斌 许瑞荣[1] 吴茂盛[1] Wei Fangfang;Li Wenli;Zhen Limin;Liao Changbin;Xu Ruirong;Wu Maosheng(Department of Infectious Disease,Guangdong Second Provincial General Hospital,Guangzhou 510317,China)

机构地区：[1]广东省第二人民医院感染内科

出　　处：《新医学》2020年第2期143-145,共3页Journal of New Medicine

摘　　要：目的探讨HBV前C(PC)区和基本核心启动子(BCP)区与乙型肝炎患者病情进展的关系。方法选择37例慢性乙型肝炎患者(慢乙肝组)及27例乙型肝炎肝硬化患者(肝硬化组),分析2组患者的一般资料和临床特征,对2组患者血清样本进行A1762T/G1764A、G1896A基因突变检测,比较组间差异。结果肝硬化组患者的年龄大于慢乙肝组,ALT、HBV DNA水平低于慢乙肝组,层粘连蛋白水平高于慢乙肝组(P均<0.05)。肝硬化组患者A1762T/G1764A双位点突变发生率为78%,高于慢乙肝组的49%(P<0.05);肝硬化组G1896A突变发生率为48%,慢乙肝组为60%,2组比较差异无统计学意义(P>0.05)。结论乙肝肝硬化患者HBV PC/BCP区A1762T/G1764A突变率高于慢乙肝患者,A1762T/G1764A突变可能参与慢乙肝发展至肝硬化的过程。Objective To explore the correlation between the gene mutations in hepatitis B virus(HBV) pre-core(PC) and basic core promoter(BCP) and the progression of hepatitis B.Methods A total of 37 patients with chronic hepatitis B(CHB) and 27 patients with CHB-induced liver cirrhosis were recruited into the CHB and liver cirrhosis groups.Baseline data and clinical characteristics were analyzed in two groups.The gene mutations of A1762 T/G1764A and G1896A in the serum samples were detected and compared between two groups.Results The age of liver cirrhosis patients was signif icantly older,the ALT and HBV DNA levels were considerably lower and the laminin level was significantly higher compared with those in the CHB group(all P<0.05).The incidence rate of of A1762T/G1764A double mutations in the liver cirrhosis group was 78%,significantly higher than 49% in CHB patients(P<0.05).The incidence rate of G1896A mutation did not significantly differ between the CHB and liver cirrhosis groups(60% vs.48%,P>0.05).Conclusions The prevalence of A1762T/G1764A double mutations in the PC/BCP of patients with liver cirrhosis is significantly higher than that in CHB patients.A1762T/G1764A mutation probably participates in the progression from CHB into liver cirrhosis.

关 键 词：慢性乙型肝炎 乙肝肝硬化 前C区突变 基本核心启动子区突变 

分 类 号：R512.62[医药卫生—内科学] R575.2[医药卫生—临床医学]