基于响应面法的山楂果核总黄酮闪式提取工艺  

Flash extraction of flavonoids from hawthorn seed using response surface methodology

作　　者：杨燕新[1] 邱相坡 于欢 王文斌[2] Yang Yanxin;Qiu Xiangpo;Yu Huan;Wang Wenbin(College of Arts and Sciences,Shanxi Agricultural University,Taigu 030801,China;College of Life Sciences,Shanxi Agricultural University,Taigu 030801,China)

机构地区：[1]山西农业大学文理学院,山西太谷030801 [2]山西农业大学生命科学学院,山西太谷030801

出　　处：《山西农业大学学报（自然科学版）》2020年第1期79-85,共7页Journal of Shanxi Agricultural University(Natural Science Edition)

基　　金：山西省基础研究计划(201801D121204);山西农业大学国家自然科学基金培育项目(2017GPY06);山西农业大学科技创新基金(2018yz003);山西农业大学博士科研启动基金(XB2009002)

摘　　要：[目的]通过响应面分析法对山楂果核总黄酮的闪式提取工艺条件进行优化。[方法]分别选取料液比、乙醇浓度、闪式提取时间完成单因素试验,然后通过响应面Design-Expert软件的Box-Behnken Design程序进行试验设计,优化出山楂果核总黄酮的闪式提取工艺参数,并进行验证。[结果]料液比为1∶29(g·mL-1)、乙醇浓度为49%、闪式提取时间为91 s时,山楂果核总黄酮提取率最佳,可达0.644%。[结论]本研究优化了山楂果核的闪式提取工艺参数,将为山楂果核总黄酮工业化提取奠定一定的理论基础,以期提升山楂果核资源的利用价值。[Objective]The objective of this study was to optimize the flash extraction conditions of total flavonoids in Hawthorn seeds by response surface methodology.[Methods]Each of the ratio of solid material to liquid,ethanol concentration,and extraction time was employed as single factor to determine the best extract condition with each factor,then Box-Behnken Design test was carried out to optimize the entire extraction conditions of total flavonoids from Hawthorn seed.[Results]The results indicated that the highest extraction rate(0.644%)was obtained when the ratio of solid material to liquid was 1∶29(g·mL-1),and the concentration of ethanol was 49%with the extraction time at 91s.[Conclusion]The extraction condition of flash extraction of flavonoids from Hawthorn seed was optimized in the study.The results will provide a scientific support for the industrialized extraction of flavonoid from Hawthorn seed and enhance the utilization value of Hawthorn seed resources.

关 键 词：山楂果核 总黄酮 闪式提取 响应面法 

分 类 号：R932[医药卫生—生药学]